Chuartzman Silvia G, Schuldiner Maya
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, 7610001, Israel.
Yeast. 2018 Jul;35(7):477-483. doi: 10.1002/yea.3312. Epub 2018 May 3.
In the last decade several collections of Saccharomyces cerevisiae yeast strains have been created. In these collections every gene is modified in a similar manner such as by a deletion or the addition of a protein tag. Such libraries have enabled a diversity of systematic screens, giving rise to large amounts of information regarding gene functions. However, often papers describing such screens focus on a single gene or a small set of genes and all other loci affecting the phenotype of choice ('hits') are only mentioned in tables that are provided as supplementary material and are often hard to retrieve or search. To help unify and make such data accessible, we have created a Database of High Throughput Screening Hits (dHITS). The dHITS database enables information to be obtained about screens in which genes of interest were found as well as the other genes that came up in that screen - all in a readily accessible and downloadable format. The ability to query large lists of genes at the same time provides a platform to easily analyse hits obtained from transcriptional analyses or other screens. We hope that this platform will serve as a tool to facilitate investigation of protein functions to the yeast community.
在过去十年中，已经创建了几个酿酒酵母菌株的集合。在这些集合中，每个基因都以类似的方式进行修饰，例如通过缺失或添加蛋白质标签。这样的文库使得各种系统筛选成为可能，产生了大量关于基因功能的信息。然而，描述此类筛选的论文通常只关注单个基因或一小部分基因，而所有其他影响所选表型（“命中基因”）的基因座仅在作为补充材料提供的表格中提及，并且往往难以检索或搜索。为了帮助统一并使这些数据易于获取，我们创建了一个高通量筛选命中基因数据库（dHITS）。dHITS数据库能够获取有关发现感兴趣基因的筛选信息以及该筛选中出现的其他基因的信息——所有这些信息都以易于访问和下载的格式呈现。同时查询大量基因列表的能力提供了一个平台，便于分析从转录分析或其他筛选中获得的命中基因。我们希望这个平台将成为促进酵母学界对蛋白质功能进行研究的工具。
