DNA 在磨牙症的昼夜节律表现中呈低甲基化状态。

DNA is hypomethylated in circadian manifestations of bruxism.

机构信息

Centro de Biología Molecular & Farmacogenética, Departamento de Ciencias Básicas, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile.

Departamento de Odontología Adultos, Facultad de Odontología, Universidad de La Frontera, Temuco, Chile.

出版信息

Oral Dis. 2018 Sep;24(6):1132-1139. doi: 10.1111/odi.12856. Epub 2018 Jun 7.

DOI:10.1111/odi.12856

PMID:29575322

Abstract

OBJECTIVE

The aim of this study was to compare the global DNA methylation levels in patients under bruxism treatment and a control group.

METHODS

Subjects undergoing bruxism treatment were classified in awake bruxism (42 patients), sleep bruxism (32 patients) and both conditions (42 patients). The control group included 42 individuals. A colorimetric assay (MethylFlash Methylated DNA 5-mC Quantification Kit, Epigenetic Group Inc., NY, USA) was used to determine the global DNA methylation levels.

RESULTS

Statistically significant differences were found in amounts of methylated DNA in all circadian manifestations of bruxism compared with a control group (sleep bruxism = 0.95% ± 2.02%; awake bruxism = 0.87% ± 2.1%; sleep and awake bruxism = 0.17% ± 0.25%; Control = 1.69% ± 1.6%; Kruskal-Wallis test [p = .0001] followed by Dunn's test [p < .05]).

CONCLUSION

Patients undergoing bruxism treatment exhibited hypomethylated DNA levels when compared to control group. Our results suggest that DNA hypomethylation might be a novel aetiologic factor in bruxism aetiology. Further researches must be performed exploring the role of epigenetics modifications in circadian manifestations of bruxism.

摘要

目的

本研究旨在比较磨牙症治疗患者和对照组的全基因组 DNA 甲基化水平。

方法

将磨牙症治疗患者分为觉醒性磨牙症（42 例）、睡眠性磨牙症（32 例）和两种情况同时存在（42 例）。对照组包括 42 名个体。采用比色法（MethylFlash Methylated DNA 5-mC Quantification Kit，Epigenetic Group Inc.，NY，USA）测定全基因组 DNA 甲基化水平。

结果

与对照组相比，所有磨牙症昼夜节律表现中甲基化 DNA 含量均存在统计学差异（睡眠性磨牙症=0.95%±2.02%；觉醒性磨牙症=0.87%±2.1%；睡眠和觉醒性磨牙症=0.17%±0.25%；对照组=1.69%±1.6%；Kruskal-Wallis 检验[P=0.0001]，随后 Dunn 检验[P<0.05]）。