可转座噬菌体D108的右端包含一段520个碱基对的蛋白质编码序列，该序列在噬菌体Mu中不存在。

The right end of transposable bacteriophage D108 contains a 520 base pair protein-encoding sequence not present in bacteriophage Mu.

作者信息

Szatmari G B, Lapointe M, DuBow M S

出版信息

Nucleic Acids Res. 1987 Aug 25;15(16):6691-704. doi: 10.1093/nar/15.16.6691.

DOI:10.1093/nar/15.16.6691

PMID:2957646

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC306132/

Abstract

We have cloned and characterized the right end terminal 796 bp of the transposable Mu-like bacteriophage D108. This region encompasses a 520 bp region of D108-specific sequences not present in phage Mu that contain an open reading frame encoding a 12 KDa protein. This protein can be visualized in vivo when the region is placed downstream from the strong lac UV5 promoter. The open reading frame can be expressed from the dam-regulated mod promoter (for modification of D108 DNA), yet also contains its own dam-independent promoter for expression that is detectable by northern blot analysis late in the D108 lytic cycle. Comparison of this region of D108 DNA with the corresponding region of Mu DNA suggests that a complex rearrangement has occurred at the phages' right ends during their evolution.

摘要

我们已经克隆并鉴定了可转座的类Mu噬菌体D108右端796 bp的序列。该区域包含一段520 bp的D108特异性序列，此序列在噬菌体Mu中不存在，其中含有一个编码12 kDa蛋白的开放阅读框。当该区域置于强lac UV5启动子下游时，这种蛋白质在体内可见。该开放阅读框可从受dam调控的mod启动子（用于D108 DNA的修饰）表达，但也含有自身的不依赖dam的启动子用于表达，在D108裂解周期后期通过Northern印迹分析可检测到。将D108 DNA的该区域与Mu DNA的相应区域进行比较表明，在噬菌体进化过程中，其右端发生了复杂的重排。