The Standardized Extract of Alleviates Chronic Alcoholic Liver Injury in C57Bl/6J Mice.

BACKGROUND

In traditional folk medicine, is used in the treatment of uterine hemorrhage, tinnitus, and oligomenorrhea.

OBJECTIVE

This study aimed to identify the therapeutic effect of EtOAc extract (EALT) on liver of mice with chronic alcohol poisoning.

MATERIALS AND METHODS

C57BL/6J mice were administered 100 mg/kg of EALT with a single binge ethanol/Lieber-DeCarli liquid diet for 8 weeks.

RESULTS

The chronic-binge ethanol diet induced a significant increase in liver marker enzyme activities. Coadministration of EALT reversed the elevation of serum total cholesterol and triglyceride as well as aspartate aminotransferase and alanine aminotransferase due to chronic alcohol consumption. Histologic findings including markedly attenuated fat accumulation in hepatocytes were observed in EALT-treated mice. EALT supplementation prevented alcoholic liver injury through attenuation of inflammatory mediators such as toll-like receptor-4, cytochrome P4502E1, and cyclooxygenase-2, and inflammatory cytokine interleukin-6.

CONCLUSION

Results provided direct experimental evidence for the hepatoprotective effect of EALT in the NIAAA mouse model. Therapeutic attempts with the extract might be useful in the management of alcoholic liver disease.

SUMMARY

Halophyte has recently been of interest in Korea for its nutritional value and salty taste which made it an ideal vegetablePhytochemical analysis of EtOAc extract (EALT) resulted in nine compounds including catechins and myricetin glycosides as main componentsAdministration of EALT for 8 weeks showed hepatoprotective effect on Lieber-DeCarli diet-fed mouse modelA significant decrease in liver marker enzymes and inflammatory mediators was also detected. EALT: EtOAc extract; TC: Total cholesterol; TG: Triglyceride; ROS: Reactive oxygen species; CYP2E1: Cytochrome P4502E1; TLR-4: Toll-like receptor-4; COX-2: Cyclooxygenase-2.