School of Pharmacy, Jeonbuk National University, Jeonju 54896, Jeonbuk, Korea.
Department of Biochemistry and Research Institute for Endocrine Sciences, Jeonbuk National University Medical School, Jeonju 54896, Jeonbuk, Korea.
Nutrients. 2022 Sep 21;14(19):3904. doi: 10.3390/nu14193904.
The purpose of this study was to examine whether , cultivated in a smart-farming system with LED lamps, could increase exercise capacity in mice. C57BL/6 male mice were orally administered vehicle or water extract (LTE), either 30 or 100 mg/kg, and were subjected to moderate intensity treadmill exercise for 4 weeks. Running distance markedly increased in the LTE group (100 mg/kg) by 80 ± 4% compared to the vehicle group, which was accompanied by a higher proportion of oxidative fibers (6 ± 6% vs. 10 ± 4%). Mitochondrial DNA content and gene expressions related to mitochondrial biogenesis were significantly increased in LTE-supplemented gastrocnemius muscles. At the molecular level, the expression of PGC-1α, a master regulator of fast-to-slow fiber-type transition, was increased downstream of the PKA/CREB signaling pathway. LTE induction of the PKA/CREB signaling pathway was also observed in C2C12 cells, which was effectively suppressed by PKA inhibitors H89 and Rp-cAMP. Altogether, these findings indicate that LTE treatment enhanced endurance exercise capacity via an improvement in mitochondrial biosynthesis and the increases in the formation of oxidative slow-twitch fibers. Future study is warranted to validate the exercise-enhancing effect of LTE in the human.
本研究旨在探讨在 LED 灯智能农业系统中培养的 是否能提高小鼠的运动能力。将 C57BL/6 雄性小鼠经口给予 vehicle 或水提取物（LTE），剂量为 30 或 100mg/kg，并进行 4 周中等强度的跑步机运动。与 vehicle 组相比，LTE（100mg/kg）组的跑步距离显著增加了 80±4%，并且氧化纤维的比例更高（6±6%对 10±4%）。LTE 补充的比目鱼肌中线粒体 DNA 含量和与线粒体生物发生相关的基因表达显著增加。在分子水平上，快速型向慢型纤维转换的主要调节因子 PGC-1α 的表达在 PKA/CREB 信号通路的下游增加。在 C2C12 细胞中也观察到 LTE 诱导的 PKA/CREB 信号通路，PKA 抑制剂 H89 和 Rp-cAMP 可有效抑制该通路。总之，这些发现表明 LTE 处理通过改善线粒体生物合成和增加氧化型慢肌纤维的形成来增强耐力运动能力。需要进一步的研究来验证 LTE 在人体中的增强运动效果。
