Pirzadeh-Ashraf Ahmad, Lotfi Mehrdad, Zarandi Ali, Yazdani Ebrahim, Mozafari Aysan, Pornasrollah Alireza
Department of Endodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Periodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Dent Res J (Isfahan). 2018 Mar-Apr;15(2):146-149.
Mineral trioxide aggregate (MTA) has a high biocompatibility and its physical properties could be improved by adding the containing silica fume an amorphous silicon dioxide (condensed silica fume [CSF]). The aim of this study was to evaluate the cytotoxicity of MTA mixed with CSF on the viability of L929 mouse fibroblast cell using 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide reduction assay (MTT assay).
In this study white MTA was mixed with distilled water according to the manufacturer's instructions. Mixtures of White MTA with 10%, 15%, and 20% CSF by weight were prepared and mixed with distilled water. Cytotoxicity of mixtures was compared with MTT assay on L929 mouse fibroblast cell line after 24, 48, and 72 h. Differences in cytotoxicity were assessed by one-way analysis of variance (ANOVA).
Mean ± SD of vital cell counts cultured in MTA, MTA + 10% CSF, MTA + 15% CSF, and MTA + 20% CSF were 98% ± 6%, 97% ± 6%, 94% ± 4%, and 98% ± 4%, respectively. One-way ANOVA did not reveal any statistically significant difference between the groups ( > 0.05).
It may be concluded that addition of CSF to MTA may not influence its cytotoxicity.
三氧化矿物凝聚体（MTA）具有高生物相容性，通过添加含有硅灰（一种无定形二氧化硅，即微硅粉[CSF]）可改善其物理性能。本研究的目的是使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐还原法（MTT法）评估与CSF混合的MTA对L929小鼠成纤维细胞活力的细胞毒性。
在本研究中，白色MTA按照制造商的说明与蒸馏水混合。制备白色MTA与10%、15%和20%（重量）CSF的混合物，并与蒸馏水混合。在24、48和72小时后，通过MTT法在L929小鼠成纤维细胞系上比较混合物的细胞毒性。通过单因素方差分析（ANOVA）评估细胞毒性的差异。
在MTA、MTA + 10% CSF、MTA + 15% CSF和MTA + 20% CSF中培养的活细胞计数的平均值±标准差分别为98% ± 6%、97% ± 6%、94% ± 4%和98% ± 4%。单因素方差分析未显示各组之间有任何统计学上的显著差异（> 0.05）。
可以得出结论，向MTA中添加CSF可能不会影响其细胞毒性。
