Pintado Laura Siqueira, Torre Eliana do Nascimento, Dos Santos Selayaran Maicon, de Carvalho Rodrigo Varella, Zanchi Cesar Henrique, Manzolli Leite Fábio Renato, Etges Adriana
Department of Semiology and Clinics, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil.
Department of Restorative Dentistry, School of Dentistry, IMED Faculdade Meridional, Passo Fundo, RS, Brazil.
J Conserv Dent. 2018 Jan-Feb;21(1):74-79. doi: 10.4103/JCD.JCD_393_16.
The purpose of this study is to compare the physical, mechanical, and biocompatibility properties of a new dual-cure white mineral trioxide aggregate (D-W-MTA) and a commercial W-MTA.
Diametral tensile strength (DTS), water sorption (WSp), and water solubility (WSl) tests were performed. Cytotoxicity was observed in primary culture of human pulp fibroblasts (HPFs) and mouse 3T3/NIH fibroblast lineage. Specimens of both materials were embedded in 1 mL of Dulbecco's modified essential medium for 24 h. Cells were incubated for 24 h with the eluates. Cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and genotoxicity by micronucleus (MN) formation. Data were analyzed by ANOVA and Kruskal-Wallis tests considering < 0.05.
D-MTA and W-MTA not showed cytotoxic effect on the two cell lines. However, D-MTA stimulated HPF growth. The MN count was similar to that of the control group for D-MTA and W-MTA. D-MTA presented lower DTS and WSl. Nevertheless, WSp was similar in the two groups.
The results suggest that D-MTA is a promising material for pulp capping. Thus, tests should be performed to evaluate the performance of this material.
本研究旨在比较一种新型双固化白色矿物三氧化物凝聚体（D-W-MTA）与市售W-MTA的物理、机械和生物相容性特性。
进行了径向拉伸强度（DTS）、吸水率（WSp）和水溶性（WSl）测试。在人牙髓成纤维细胞（HPFs）和小鼠3T3/NIH成纤维细胞系的原代培养中观察细胞毒性。将两种材料的样本嵌入1 mL Dulbecco改良的基本培养基中24小时。将细胞与洗脱液孵育24小时。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐试验评估细胞毒性，通过微核（MN）形成评估遗传毒性。考虑P<0.05，采用方差分析和Kruskal-Wallis检验对数据进行分析。
D-MTA和W-MTA对两种细胞系均未显示细胞毒性作用。然而，D-MTA刺激了HPF的生长。D-MTA和W-MTA的MN计数与对照组相似。D-MTA的DTS和WSl较低。然而，两组的WSp相似。
结果表明D-MTA是一种有前景的盖髓材料。因此，应进行测试以评估该材料的性能。
