Halvorsen R, Gaudernack G, Leivestad T, Vartdal F, Thorsby E
Scand J Immunol. 1987 Aug;26(2):197-205. doi: 10.1111/j.1365-3083.1987.tb02252.x.
We studied the requirements for secondary activation signals in pure CD4+ and CD8+ T cells after stimulation with anti-CD3 antibodies. Stimulation of CD4+ or CD8+ cells with anti-CD3 monoclonal antibodies (MoAb) bound to polystyrene monosized particles never resulted in a proliferative response. However, DNA synthesis was observed when recombinant interleukin 2 (IL-2) or other secondary signals, such as those provided by phorbol myristate acetate (PMA) or autologous accessory cells (AC), were also added. These secondary signals were not in themselves capable of inducing DNA synthesis in the absence of particle-bound anti-CD3. We also found that the signals provided by AC may be dependent on the activation state of these cells. Thus, the effects of accessory cells were enhanced by a factor present in fetal calf serum (FCS), most likely endotoxin or lipopolysaccharide (LPS), which alone, however, were not able to activate T cells, even in the presence of particle-bound anti-CD3. Recombinant IL-1 over a broad dose range was unable to replace PMA or activated AC after stimulation with particle-bound anti-CD3. Purified CD4+ and CD8+ T cells behaved identically in all the experiments, indicating that the basic mechanisms for activation in the two T-cell subsets are identical.
我们研究了用抗 CD3 抗体刺激后，纯 CD4+ 和 CD8+ T 细胞中二次激活信号的需求。用结合于聚苯乙烯单尺寸颗粒的抗 CD3 单克隆抗体（MoAb）刺激 CD4+ 或 CD8+ 细胞，从未产生增殖反应。然而，当添加重组白细胞介素 2（IL-2）或其他二次信号，如佛波酯肉豆蔻酸酯乙酸盐（PMA）或自体辅助细胞（AC）提供的信号时，可观察到 DNA 合成。在没有颗粒结合的抗 CD3 的情况下，这些二次信号本身不能诱导 DNA 合成。我们还发现，辅助细胞提供的信号可能取决于这些细胞的激活状态。因此，胎牛血清（FCS）中存在的一种因子可增强辅助细胞的作用，最有可能是内毒素或脂多糖（LPS），然而，单独的内毒素或脂多糖即使在存在颗粒结合的抗 CD3 的情况下也不能激活 T 细胞。在与颗粒结合的抗 CD3 刺激后，宽剂量范围内的重组 IL-1 不能替代 PMA 或活化的辅助细胞。在所有实验中，纯化的 CD4+ 和 CD8+ T 细胞表现相同，表明两个 T 细胞亚群激活的基本机制相同。
