Halvorsen R, Leivestad T, Gaudernack G, Thorsby E
Institute of Transplantation Immunology, National Hospital, Rikshospitalet, Oslo, Norway.
Scand J Immunol. 1988 May;27(5):555-63. doi: 10.1111/j.1365-3083.1988.tb02383.x.
The ability of different subpopulations of blood mononuclear cells to serve as accessory cells in the activation of CD4+ and CD8+ T cells via Ti-CD3 or with phytohaemagglutinin (PHA) was studied. Pure CD4+ or CD8+ T cells did not respond to particle-bound anti-CD3 monoclonal antibodies (MoAb) or PHA, whereas responses were seen when non-T cells served as accessory cells. Removal of class II-positive cells from peripheral blood mononuclear cells (PBMC) or from non-T cells diminished, but did not completely abolish, the responses in both T cell subsets, indicating that the accessory cells are mainly found among the class II-positive cells. However, the class II molecules themselves were not involved, as demonstrated in antibody-blocking experiments. Removal of monocytes decreased the ability of non-T cells to serve as accessory cells for both CD4+ and CD8+ cells in PHA activation. In contrast, the removal of monocytes resulted in an enhanced activation by anti-CD3 MoAb in CD4+ T cells, while the activation of CD8+ T cells was less affected. Positively selected B cells were effective accessory cells in anti-CD3 and PHA activation. Furthermore, Epstein-Barr virus (EBV)-transformed B cell lines were very potent accessory cells both in anti-CD3 and PHA activation of T cells, and showed the strongest accessory cell function observed in this system on a per cell basis.
研究了血液单核细胞不同亚群作为辅助细胞通过Ti-CD3或植物血凝素（PHA）激活CD4+和CD8+T细胞的能力。纯CD4+或CD8+T细胞对颗粒结合的抗CD3单克隆抗体（MoAb）或PHA无反应，而当非T细胞作为辅助细胞时则可见反应。从外周血单核细胞（PBMC）或非T细胞中去除II类阳性细胞会减弱，但不会完全消除两个T细胞亚群中的反应，这表明辅助细胞主要存在于II类阳性细胞中。然而，如抗体阻断实验所示，II类分子本身并不参与。去除单核细胞会降低非T细胞在PHA激活中作为CD4+和CD8+细胞辅助细胞的能力。相反，去除单核细胞会增强抗CD3 MoAb对CD4+T细胞的激活，而CD8+T细胞的激活受影响较小。阳性选择的B细胞在抗CD3和PHA激活中是有效的辅助细胞。此外，爱泼斯坦-巴尔病毒（EBV）转化的B细胞系在T细胞的抗CD3和PHA激活中都是非常有效的辅助细胞，并且在该系统中按每个细胞计算显示出最强的辅助细胞功能。
