Cuq Benoît, Blois Shauna L, Wood R Darren, Monteith Gabrielle, Abrams-Ogg Anthony C, Bédard Christian, Wood Geoffrey A
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
Vet Clin Pathol. 2018 Jun;47(2):218-226. doi: 10.1111/vcp.12602. Epub 2018 Mar 26.
Thrombin plays a central role in hemostasis and thrombosis. Calibrated automated thrombography (CAT), a thrombin generation assay, may be a useful test for hemostatic disorders in dogs.
To describe CAT results in a group of healthy dogs, and assess preanalytical variables and biological variability.
Forty healthy dogs were enrolled.
Lag time (Lag), time to peak (ttpeak), peak thrombin generation (peak), and endogenous thrombin potential (ETP) were measured. Direct jugular venipuncture and winged-needle catheter-assisted saphenous venipuncture were used to collect samples from each dog, and results were compared between methods. Sample stability at -80°C was assessed over 12 months in a subset of samples. Biological variability of CAT was assessed via nested ANOVA using samples obtained weekly from a subset of 9 dogs for 4 consecutive weeks.
Samples for CAT were stable at -80°C over 12 months of storage. Samples collected via winged-needle catheter venipuncture showed poor repeatability compared to direct venipuncture samples; there was also poor agreement between the 2 sampling methods. Intra-individual variability of CAT parameters was below 25%; inter-individual variability ranged from 36.9% to 78.5%.
Measurement of thrombin generation using CAT appears to be repeatable in healthy dogs, and samples are stable for at least 12 months when stored at -80°C. Direct venipuncture sampling is recommended for CAT. Low indices of individuality suggest that subject-based reference intervals are more suitable when interpreting CAT results.
凝血酶在止血和血栓形成中起核心作用。校准自动血栓形成测定法（CAT）作为一种凝血酶生成测定方法，可能是检测犬类止血障碍的有用测试。
描述一组健康犬的CAT结果，并评估分析前变量和生物学变异。
纳入40只健康犬。
测量滞后时间（Lag）、达到峰值的时间（ttpeak）、凝血酶生成峰值（peak）和内源性凝血酶潜力（ETP）。使用直接颈静脉穿刺和翼针导管辅助的隐静脉穿刺从每只犬采集样本，并比较两种方法的结果。在一部分样本中评估样本在-80°C下12个月的稳定性。通过嵌套方差分析，使用从9只犬的一个子集连续4周每周获得的样本评估CAT的生物学变异。
CAT样本在-80°C下储存12个月稳定。与直接静脉穿刺样本相比，通过翼针导管静脉穿刺采集的样本重复性较差；两种采样方法之间的一致性也较差。CAT参数的个体内变异低于25%；个体间变异范围为36.9%至78.5%。
在健康犬中，使用CAT测量凝血酶生成似乎具有可重复性，样本在-80°C储存时至少12个月稳定。建议CAT采用直接静脉穿刺采样。低个体性指数表明，在解释CAT结果时，基于个体的参考区间更合适。
