Faculty of Biotechnology, University of Wroclaw, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland.
Faculty of Biotechnology, University of Wroclaw, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland; Department of Genetics, Plant Breeding and Seed Production, Faculty of Life Sciences and Technology, Wroclaw University of Environmental and Plant Sciences, Plac Grunwaldzki 24A, 53-363 Wroclaw, Poland.
Plant Physiol Biochem. 2018 Jun;127:143-151. doi: 10.1016/j.plaphy.2018.03.023. Epub 2018 Mar 21.
Previously we described flax plants with expression of Arabidopsis lycopene β-cyclase (lcb) gene in which decreased expression of the endogenous lcb and increased resistance to fungal pathogen was observed. We suggested that co-suppression was responsible for the change. In this study we investigated the molecular basis of the observed effect in detail. We found that methylation changes in the Lulcb gene body might be responsible for repression of the gene. Treatment with azacitidine (DNA methylation inhibitor) confirmed the results. Moreover, we studied how the manipulation of carotenoid biosynthesis pathway increased ABA level in these plants. We suggest that elevated ABA levels may be responsible for the increased resistance of the flax plants to pathogen infection through activation of chitinase (PR gene).
先前，我们描述了拟南芥番茄红素 β-环化酶(lcb)基因在亚麻植株中的表达情况，在这些植株中观察到内源性 lcb 的表达降低和对真菌病原体的抗性增加。我们推测这是共抑制作用的结果。在这项研究中，我们详细研究了观察到的效果的分子基础。我们发现，Lulcb 基因体中的甲基化变化可能是导致基因沉默的原因。用阿扎胞苷（DNA 甲基化抑制剂）处理证实了这一结果。此外，我们还研究了类胡萝卜素生物合成途径的操纵如何增加这些植物中的 ABA 水平。我们认为，ABA 水平的升高可能通过激活几丁质酶（PR 基因）而导致亚麻植株对病原体感染的抗性增加。
