Department of Neurosurgery, Second Affiliated Hospital, Soochow University, Suzhou, 215004, 1055 Sanxiang Road, Jiangsu Province, China.
Department of Neurosurgery, Second Affiliated Hospital, Soochow University, Suzhou, 215004, 1055 Sanxiang Road, Jiangsu Province, China.
Biomed Pharmacother. 2018 Jun;102:452-457. doi: 10.1016/j.biopha.2018.03.083. Epub 2018 Apr 5.
It has been certified that long noncoding RNAs (lncRNAs) are crucial regulators in the progression of various human cancers. snoRNA host gene 6 (SNHG6) has been uncovered to affect the initial stage and tumorigenesis of hepatocellular carcinoma. Nevertheless, the expression pattern and biological role of SNHG6 in glioma still need to be investigated. The study aims to investigate the expression pattern, biological role and the potential mechanism of SNHG6 in glioma. In this study, the high expression of SNHG6 was tested in both glioma tissues and glioma cells. The correlation between expression levels of SNHG6 and the overall survival of glioma patients was demonstrated by using Kaplan Meier method analysis. Next, gain of function assays revealed that overexpression of SNHG6 can promote the formation of malignant phenotype of 1800 cell. However, results of loss-of-function assays revealed that silenced SNHG6 exerted the inhibitory function on glioma cell growth. Flow cytometric analysis was performed in glioma cells to detect the anti-oncogenic effects of silenced SNHG6 on cell cycle and apoptosis. Finally, we identified that p21 was involved in glioma cell proliferation after SNHG6 was downregulated. Taken together, we concluded that SNHG6 is a regulator and a potential therapeutic target in glioma.
已有研究证明，长链非编码 RNA（lncRNA）在多种人类癌症的进展中是至关重要的调控因子。snoRNA 宿主基因 6（SNHG6）已被发现影响肝癌的早期阶段和肿瘤发生。然而，SNHG6 在神经胶质瘤中的表达模式和生物学作用仍需进一步研究。本研究旨在探讨 SNHG6 在神经胶质瘤中的表达模式、生物学作用及其潜在机制。在本研究中，检测了神经胶质瘤组织和神经胶质瘤细胞中 SNHG6 的高表达。通过 Kaplan-Meier 方法分析表明，SNHG6 的表达水平与神经胶质瘤患者的总生存率相关。接下来，功能获得实验表明，SNHG6 的过表达可以促进 1800 细胞恶性表型的形成。然而，功能丧失实验的结果表明，沉默 SNHG6 对神经胶质瘤细胞的生长具有抑制作用。通过流式细胞术分析检测沉默 SNHG6 对细胞周期和凋亡的抗肿瘤作用。最后，我们确定在 SNHG6 下调后，p21 参与了神经胶质瘤细胞的增殖。综上所述，我们得出结论，SNHG6 是神经胶质瘤的调控因子和潜在的治疗靶点。
