State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
Laboratory of Phytopathology, University of Liège, Gembloux Agro-BioTech, Passage des déportés, 2, 5030 Gembloux, Belgium.
J Gen Virol. 2018 May;99(5):710-716. doi: 10.1099/jgv.0.001052. Epub 2018 Mar 26.
There is little information about commensal viruses in the white-backed planthopper, Sogatella furcifera, although it is an important agricultural insect. Here, two novel double-stranded RNA viruses related to the viruses in the family Totiviridae were identified using next-generation sequencing and tentatively named Sogatella furcifera totivirus 1 and 2 (SfTV1 and SfTV2). Their complete genomes consist of 6310 and 6303 nt, respectively, showing typical genomic features with viruses in the family Totiviridae. Identity, phylogenetic and conserved sequence analyses showed that SfTV1, SfTV2 and three other insect viruses may form a proposed novel genus of the family Totiviridae. Vertical transmission of the two viruses was highly efficient, and they were detected in all insect tissues and developmental stages, with the highest titres in the adult and in the haemolymph and reproductive organs. To our knowledge, this is the first report of viruses in the family Totiviridae found in a hemipteran insect.
关于白背飞虱体内的共生病毒的信息较少，尽管它是一种重要的农业昆虫。在这里，我们使用下一代测序技术鉴定出两种与 Totiviridae 家族病毒相关的新型双链 RNA 病毒，并暂命名为 Sogatella furcifera totivirus 1 和 2 (SfTV1 和 SfTV2)。它们的完整基因组分别由 6310 和 6303 个核苷酸组成，显示出与 Totiviridae 家族病毒的典型基因组特征。同源性、系统发育和保守序列分析表明，SfTV1、SfTV2 和另外三种昆虫病毒可能形成 Totiviridae 家族的一个新提议属。这两种病毒的垂直传播效率非常高，它们在所有昆虫组织和发育阶段都有检测到，在成虫和血淋巴以及生殖器官中的滴度最高。据我们所知，这是首次在半翅目昆虫中发现 Totiviridae 家族的病毒。
