Xiao Jiajing, Yang Guang, Liu Renyi, Ge Danfeng
Center for Agroforestry Mega Data Science, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Insects. 2024 Oct 6;15(10):772. doi: 10.3390/insects15100772.
(Cicadellidae: Deltocephalinae), a leafhopper prevalent in tropical and temperate regions, is notably abundant in grasses and rice. The virome of was investigated using deep transcriptome sequencing, leading to the first identification of four viruses belonging to the families , , and in . These viruses have been provisionally named B. incisa ollusvirus 1 (BiOV1), B. incisa ollusvirus 2 (BiOV2), B. incisa iflavirus 1 (BiIV1), and B. incisa totivirus 1 (BiTV1). The complete genome sequences of these viruses were obtained through rapid amplification of cDNA ends (RACE). BiOV1 has a linear genome of 15,125 nucleotides (nt), while BiOV2 possesses a circular genome of 14,853 nt. The BiIV1 genome, excluding the poly(A) tail, is 10,903 nt in length and encodes a single open reading frame (ORF) for a polyprotein consisting of 3194 amino acids (aa). The BiTV1 genome is 4357 nt long and contains two overlapping ORFs, with the viral RNA-dependent RNA polymerase (RdRp) translated via a -1 ribosomal frameshift. Phylogenetic and sequence identity analyses suggest that all these viruses are novel members of their respective families. This study significantly expands our understanding of the virome associated with by reporting and characterizing these novel viruses.
（叶蝉科：小叶蝉亚科）是一种在热带和温带地区普遍存在的叶蝉，在禾本科植物和水稻中尤为常见。利用深度转录组测序对其病毒组进行了研究，首次在该叶蝉中鉴定出四种属于不同科的病毒。这些病毒被暂时命名为水稻普通矮缩病毒1（BiOV1）、水稻普通矮缩病毒2（BiOV2）、水稻黄化斑驳病毒1（BiIV1）和水稻双链RNA病毒1（BiTV1）。通过cDNA末端快速扩增（RACE）获得了这些病毒的完整基因组序列。BiOV1有一个15125个核苷酸（nt）的线性基因组，而BiOV2有一个14853 nt的环状基因组。BiIV1基因组（不包括poly(A)尾）长度为10903 nt，编码一个由3194个氨基酸（aa）组成的多聚蛋白的单一开放阅读框（ORF）。BiTV1基因组长度为4357 nt，包含两个重叠的ORF，病毒RNA依赖性RNA聚合酶（RdRp）通过-1核糖体移码进行翻译。系统发育和序列同一性分析表明，所有这些病毒都是各自科的新成员。这项研究通过报道和表征这些新病毒，显著扩展了我们对与该叶蝉相关的病毒组的认识。
