Wallenberg Wood Science Center, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden; Department of Microtechnology and Nanoscience, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
Wallenberg Wood Science Center, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden; Department of Chemistry and Chemical Engineering, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
Carbohydr Polym. 2018 Jun 1;189:22-30. doi: 10.1016/j.carbpol.2018.01.097. Epub 2018 Feb 1.
Neural tissue engineering (TE), an innovative biomedical method of brain study, is very dependent on scaffolds that support cell development into a functional tissue. Recently, 3D patterned scaffolds for neural TE have shown significant positive effects on cells by a more realistic mimicking of actual neural tissue. In this work, we present a conductive nanocellulose-based ink for 3D printing of neural TE scaffolds. It is demonstrated that by using cellulose nanofibrils and carbon nanotubes as ink constituents, it is possible to print guidelines with a diameter below 1 mm and electrical conductivity of 3.8 × 10 S cm. The cell culture studies reveal that neural cells prefer to attach, proliferate, and differentiate on the 3D printed conductive guidelines. To our knowledge, this is the first research effort devoted to using cost-effective cellulosic 3D printed structures in neural TE, and we suppose that much more will arise in the near future.
神经组织工程（TE）是一种创新性的生物医学脑研究方法，非常依赖于支架，支架支持细胞发育成功能性组织。最近，用于神经 TE 的 3D 图案化支架通过更真实地模拟实际神经组织，对细胞产生了显著的积极影响。在这项工作中，我们提出了一种基于导电纳米纤维素的墨水，用于 3D 打印神经 TE 支架。结果表明，通过使用纤维素纳米纤维和碳纳米管作为墨水成分，可以打印直径低于 1mm 且电导率为 3.8×10-1 S cm-1 的线条。细胞培养研究表明，神经细胞更喜欢在 3D 打印的导电导线上附着、增殖和分化。据我们所知，这是首次致力于在神经 TE 中使用具有成本效益的纤维素 3D 打印结构的研究，我们预计在不久的将来会有更多的研究。
