CERTBIO, Unidade Académica de Engenharia dos Materiais, Universidade Federal de Campina Grande, Av. Aprígio Veloso 882, 58429-200 Campina Grande, Paraíba, Brazil.
Unidade Académica de Engenharia de Alimentos, Universidade Federal de Campina Grande, Av. Aprígio Veloso 882, 58429-200 Campina Grande, Paraíba, Brazil.
Carbohydr Polym. 2018 Jun 1;189:238-249. doi: 10.1016/j.carbpol.2018.02.045. Epub 2018 Feb 20.
Injectable scaffolds find many applications on the biomedical field due to several advantages on preformed scaffolds such as being able to fill any defect can be used in minimal invasion surgeries and are ready to use products. The most critical parameter for an injectable scaffold usage is its injectability, which can be related with rheological properties. Therefore, the objective of the present work was to increase knowledge about the critical parameters influencing injectability of biopolymers used for injectable scaffolds. Rheological and mechanical properties of a biopolymer blend in combination with injectability tests for a given design space controlled by the concentrations of both polymers and temperatures was made. Then those results were modeled to better understand the impact of parameters on injectability. The biopolymer blend chosen was Chitosan physically blended with Poly(ethylene glycol) where variations of both polymer concentrations and molecular weights were tested. Rheological and mechanical properties of all samples were determined, together with the injection force using a compression test at different injection conditions. All solutions were clear and transparent suggesting perfect miscibility. Rheological results were modeled using Ostwald-Waelle law and revealed a shear thinning pseudo-plastic solution at any composition and temperature, being chitosan concentration the most influencing variable. Compression tests results revealed mean injection forces ranging from 9.9 ± 0.06N to 29.9 ± 0.65N and it was possible to accurately estimate those results. Simulations revealed draw speed as the most influencing parameter. Cell viability tests revealed a non-cytotoxic biopolymer blend.
可注射支架由于具有优于预制支架的许多优点，例如能够填充任何缺陷、可用于微创手术以及是即用型产品，因此在生物医学领域有广泛的应用。对于可注射支架的使用，最关键的参数是其可注射性，这与流变性能有关。因此，本工作的目的是增加对用于可注射支架的生物聚合物的可注射性的关键参数的了解。通过流变和机械性能测试以及在给定设计空间内的可注射性测试（该设计空间由两种聚合物的浓度和温度控制）来研究生物聚合物共混物。然后对这些结果进行建模，以更好地了解参数对可注射性的影响。所选择的生物聚合物共混物是壳聚糖与聚乙二醇物理共混，其中测试了两种聚合物浓度和分子量的变化。在不同的注射条件下，通过压缩试验测定了所有样品的流变和机械性能以及注射力。所有溶液均清晰透明，表明具有完美的混溶性。使用奥斯特瓦尔德-沃尔勒定律对流变学结果进行建模，结果表明在任何组成和温度下，溶液均呈现出剪切稀化的假塑性，壳聚糖浓度是最具影响的变量。压缩试验结果表明，平均注射力范围为 9.9 ± 0.06N 至 29.9 ± 0.65N，并且可以准确估计这些结果。模拟结果表明，牵伸速度是最具影响的参数。细胞活力测试表明该生物聚合物共混物无细胞毒性。
