术前促红细胞生成素治疗可改善肝硬化大鼠模型大肝切除术后的生存率。

Preoperative erythropoietin treatment improves survival following major hepatic resection in a cirrhotic rat model.

作者信息

Suh Suk-Won, Choi Yoo Shin, Hong Soon Auck, Kang Hyun, Lee Donghyun, Kim Hye Ryoun

机构信息

Department of Surgery, College of Medicine, Chung-Ang University, 224-1, Heuk Seok-Dong, Dongjak-Ku, Seoul, 156-755, South Korea.

出版信息

Clin Res Hepatol Gastroenterol. 2018 Sep;42(4):347-352. doi: 10.1016/j.clinre.2017.12.001. Epub 2018 Mar 23.

DOI:10.1016/j.clinre.2017.12.001

PMID:29580765

Abstract

AIM

Major hepatic resection of a cirrhotic liver may result in a fatal clinical course. Preoperative erythropoietin (EPO) treatment has been shown to have protective properties and to stimulate liver regeneration. This study aims to investigate the effect of preoperative EPO on survival following major hepatic resection in a cirrhotic rat model.

METHODS

Cirrhotic liver was induced by intraperitoneal injection of thioacetamide (200mg/kg/mL) in 72 Lewis rats. Each 36 rats received EPO (1IU/g, every second day, 5 times preoperatively) or saline (control) and major hepatectomy (removal of the left and half of the median lobe) was performed. Biochemical and immunohistochemical parameters, cytokines and overall survival were compared following surgery.

RESULTS

Rats that received preoperative EPO had decreased hepatic aspartate aminotransferase, alanine aminotransferase and interleukin (IL)-1β expression, 48hours following surgery. They had increased hepatocyte growth factor and vascular endothelial growth factor expression at 1hour, increased IL-6 expression at 24, 48 and 120hours and increased Ki-67, 120hours following surgery. Overall, survival was significantly improved among EPO-treated rats (P=0.034).

CONCLUSION

Preoperative EPO treatment has a protective effect and stimulates liver regeneration, leading to improved overall survival following major hepatectomy in a cirrhotic rat model.

摘要

目的

对肝硬化肝脏进行大范围肝切除可能导致致命的临床病程。术前使用促红细胞生成素（EPO）治疗已显示出具有保护作用并能刺激肝脏再生。本研究旨在探讨术前EPO对肝硬化大鼠模型大范围肝切除术后生存情况的影响。

方法

通过腹腔注射硫代乙酰胺（200mg/kg/mL）在72只Lewis大鼠中诱导肝硬化。每组36只大鼠接受EPO（1IU/g，每隔一天，术前5次）或生理盐水（对照组），并进行大范围肝切除术（切除左叶和中叶的一半）。术后比较生化和免疫组化参数、细胞因子及总体生存率。

结果

接受术前EPO治疗的大鼠在术后48小时肝天冬氨酸转氨酶、丙氨酸转氨酶及白细胞介素（IL）-1β表达降低。它们在术后1小时肝细胞生长因子和血管内皮生长因子表达增加，在术后24、48和120小时IL-6表达增加，在术后120小时Ki-67增加。总体而言，EPO治疗组大鼠的生存率显著提高（P = 0.034）。