Cook J M, Gualde N, Hessel L, Mounier M, Michel J P, Denis F, Ratinaud M H
Groupe de Recherche en Immunologie et Biochimie, UER de Médecine, Limoges, France.
Cell Immunol. 1987 Oct 1;109(1):89-96. doi: 10.1016/0008-8749(87)90294-2.
The specific binding of hepatitis B (HBs) antigen by lymphocytes from old people immunized with hepatitis B vaccine was explored. For that purpose HBs antigen was combined with fluorescent microspheres, and labeled antigen was allowed to react with lymphocytes from HBs vaccine-responsive or unresponsive people. Lymphocytes from 10 responders and 14 nonresponders were tested for their antigen-binding ability. For controls, lymphocytes were incubated with microspheres bearing human albumin. Lymphocytes from 8 out of 10 responders were able to recognize HBs antigen; for the nonresponders the ratio was 9 out of 14. HBs-binding lymphocytes were B cells but not T lymphocytes. B and T cells from responders and nonresponders were combined and cultivated for 8 days in the presence of HBs antigen, and antibody-producing cells were counted. Neither B cells alone nor B cells plus T cells from nonresponders were able to produce antibody. On the other hand B cells from unresponsive old people produced antibodies when they were cultivated in the presence of HBs antigen and T cells from responsive old people. These data suggest that some elderly individuals who do not produce antibody after in vivo immunization by HBs vaccine do have antibody-producing cells. Instead of a gap in their immune repertoire, these people are suffering from immune dysfunction.
对接种乙肝疫苗的老年人淋巴细胞与乙肝（HBs）抗原的特异性结合进行了研究。为此，将HBs抗原与荧光微球结合，使标记抗原与对HBs疫苗有反应或无反应人群的淋巴细胞发生反应。检测了10名有反应者和14名无反应者的淋巴细胞的抗原结合能力。作为对照，淋巴细胞与携带人白蛋白的微球一起孵育。10名有反应者中有8人的淋巴细胞能够识别HBs抗原；无反应者的这一比例为14人中的9人。结合HBs的淋巴细胞是B细胞而非T淋巴细胞。将有反应者和无反应者的B细胞和T细胞混合，并在存在HBs抗原的情况下培养8天，然后对产生抗体的细胞进行计数。无反应者的B细胞单独培养或与T细胞共同培养时均不能产生抗体。另一方面，无反应老年人的B细胞在有HBs抗原存在的情况下培养，同时加入有反应老年人的T细胞时，能够产生抗体。这些数据表明，一些在体内接种HBs疫苗后不产生抗体的老年人确实具有产生抗体的细胞。这些人并非免疫 repertoire 存在缺陷，而是患有免疫功能障碍。
