Takekura H, Yoshioka T
Department of Physiology, Tokai University School of Medicine, Kanagawa, Japan.
J Muscle Res Cell Motil. 1987 Aug;8(4):342-8. doi: 10.1007/BF01568890.
Single muscle fibres separated from extensor digitorum longus (EDL) as well as soleus (SOL) in the Wistar strain male rat in relaxing solution were typed histochemically, then glycolytic and oxidative enzyme activities were determined on the same fibres. Glycolytic enzyme lactate dehydrogenase (LDH), phosphofructokinase (PFK), pyruvate kinase (PK) and creatine kinase (CK) showed highest activities in fast-twitch glycolytic (FG), lower in fast-twitch oxidative glycolytic (FOG) and lowest in slow-twitch oxidative (SO) fibres. Also oxidative enzyme succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) showed highest activities in SO, lower in FOG and lowest in FG fibres. The activities of LDH, PFK, PK and CK in FOG fibres separated from EDL showed higher activity compared to those separated from SOL, whereas the opposite result was obtained for the activities of SDH and MDH. Enzyme activities in a single muscle fibre type were not distinguishable from those of another type, and the activity profiles overlapped over a considerable range. The correlations among the separate enzyme activities of CK, LDH and MDH obtained from the same single fibre overlapped over a considerable range.
从Wistar品系雄性大鼠的趾长伸肌（EDL）和比目鱼肌（SOL）分离出的单根肌纤维，在松弛溶液中进行组织化学分型，然后测定同一纤维的糖酵解和氧化酶活性。糖酵解酶乳酸脱氢酶（LDH）、磷酸果糖激酶（PFK）、丙酮酸激酶（PK）和肌酸激酶（CK）在快肌糖酵解型（FG）纤维中活性最高，在快肌氧化糖酵解型（FOG）纤维中活性较低，在慢肌氧化型（SO）纤维中活性最低。氧化酶琥珀酸脱氢酶（SDH）和苹果酸脱氢酶（MDH）在SO纤维中活性最高，在FOG纤维中活性较低，在FG纤维中活性最低。从EDL分离出的FOG纤维中LDH、PFK、PK和CK的活性高于从SOL分离出的FOG纤维，而SDH和MDH的活性则相反。单种肌纤维类型中的酶活性与另一种类型的酶活性无法区分，且活性曲线在相当大的范围内重叠。从同一单根纤维获得的CK、LDH和MDH各自的酶活性之间的相关性在相当大的范围内重叠。
