Spamer C, Pette D
Histochemistry. 1977 Jun 8;52(3):201-16. doi: 10.1007/BF00495857.
Methods for standardized determination of phosphofructokinase (PFK), glyceraldehydephosphate dehydrogenase (GAPDH), lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) activities in nanogram samples of microdissected single fibres of rabbit psoas and soleus muscle are described. Fast and slow fibres in soleus muscle show lower absolute activities of these enzymes than the respective fibre types in psoas muscle. Slow fibres represent a more uniform population in the two muscles according to absolute and relative activities of the enzymes investigated. Slow fibres are characterized by high activities of MDH and relatively low activities of glycolytic enzymes. Fast fibres in the soleus muscle represent a population with high activities of MDH and glycolytic enzymes. Fast fibres in psoas muscle represent a heterogeneous population with high activities of glycolytic enzymes and extremely variable activity of MDH. More than 10-fold differences exist in the MDH activities of the extreme types of this fibre population. Differences in the activity levels of MDH in single fast type fibres but also in the activities of glycolytic enzymes between fast and slow fibres are greater than those reported between extreme white and red rabbit muscles.
本文描述了在兔腰大肌和比目鱼肌显微切割单纤维纳克级样本中，标准化测定磷酸果糖激酶（PFK）、甘油醛-3-磷酸脱氢酶（GAPDH）、乳酸脱氢酶（LDH）和苹果酸脱氢酶（MDH）活性的方法。比目鱼肌中的快肌纤维和慢肌纤维，其这些酶的绝对活性低于腰大肌中相应的纤维类型。根据所研究酶的绝对和相对活性，慢肌纤维在这两块肌肉中表现出更均匀的群体特征。慢肌纤维的特点是MDH活性高，糖酵解酶活性相对较低。比目鱼肌中的快肌纤维代表MDH和糖酵解酶活性高的群体。腰大肌中的快肌纤维代表糖酵解酶活性高且MDH活性变化极大的异质群体。该纤维群体极端类型的MDH活性存在超过10倍的差异。单根快肌纤维中MDH活性水平的差异，以及快肌纤维和慢肌纤维之间糖酵解酶活性的差异，大于极端白色和红色兔肌肉之间报道的差异。
