School of Life Sciences, Guangzhou University, Guangzhou 510655, PR China.
School of Life Sciences, Guangzhou University, Guangzhou 510655, PR China.
Ecotoxicol Environ Saf. 2018 Jul 30;156:403-408. doi: 10.1016/j.ecoenv.2018.03.002. Epub 2018 Mar 26.
This study aimed to determine the effects of Androstenodione (AED) on the transcriptional expression of genes involved in the hypothalamic-pituitary-gonadal (HPG) and the hypothalamic-pituitary-adrenal (HPA) axes in the zebrafish embryos/larvae. Zebrafish embryos were exposed to 0, 4.0, 45.0, 487.0, and 980.0 ng/L of AED from the day of fertilization to 144 h post fertilization (hpf), during which the transcriptional profiles of key genes related to the HPG and HPA axes were examined daily using quantitative real-time PCR. The AED exposure significantly up-regulated several receptor signaling pathways and the key genes involved in various steps of the steroidogenic pathways were also affected. In addition, the AED exposure could significantly modulate the transcriptional profiles of the other target genes related to hypothalamic and pituitary hormones. The findings of this study suggest that AED, at environmentally relevant concentrations, affects the adrenal endocrine systems and the reproduction of zebrafish by interrupting the HPG and HPA axes.
本研究旨在确定雄烷二酮（AED）对斑马鱼胚胎/幼鱼下丘脑-垂体-性腺（HPG）和下丘脑-垂体-肾上腺（HPA）轴相关基因转录表达的影响。从受精之日起，将斑马鱼胚胎暴露于 0、4.0、45.0、487.0 和 980.0ng/L 的 AED 中，直至受精后 144 小时（hpf），在此期间，每日使用定量实时 PCR 检测与 HPG 和 HPA 轴相关的关键基因的转录谱。AED 暴露显著上调了几个受体信号通路，并且类固醇生成途径中涉及的多个步骤的关键基因也受到影响。此外，AED 暴露还可以显著调节与下丘脑和垂体激素相关的其他靶基因的转录谱。本研究结果表明，AED 在环境相关浓度下通过干扰 HPG 和 HPA 轴，影响肾上腺内分泌系统和斑马鱼的繁殖。
