School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Waihuan East Road No. 232, Guangzhou Higher Education Mega Center, Guangzhou 510006, China.
Innovative Research & Development Laboratory of TCM, Guangzhou University of Chinese Medicine, Waihuan East Road No. 232, Guangzhou Higher Education Mega Center, Guangzhou 510006, China;.
Molecules. 2018 Mar 24;23(4):754. doi: 10.3390/molecules23040754.
Two 2-phenyl-benzofurans, moracin C {2-[3',5'-dihydroxy-4'-(3-methlbut-2-enyl)phenyl]-6-hydroxybenzofuran} and its isomer moracin C{2-[3',5'-dihydroxy-4'-(3-methlbut-1-enyl)phenyl]-6-hydroxybenzofuran}, were comparatively studied using redox-related antioxidant assays and non-redox antioxidant assays. Moracin C always resulted in higher IC values than -moracin C in the redox-related antioxidant assays, including •O₂-inhibition, Cu-reducing power, DPPH•-inhibition, and ABTS⁺•-inhibition assays. In the non-redox antioxidant assay, moracin C and -moracin C underwent similar radical-adduct-formation (RAF), evidenced by the peaks at 704 and 618 in HPLC-MS spectra. In conclusion, both moracin C and -moracin C can act as 2-phenyl-benzofuran antioxidants; their antioxidant mechanisms may include redox-related ET and H⁺-transfer, and non-redox RAF. A double bond at the conjugation position can enhance the redox-related antioxidant potential, but hardly affects the RAF potential.
两种 2-苯基苯并呋喃，莫拉辛 C（2-[3'，5'-二羟基-4'-(3-甲基-2-丁烯基）苯基]-6-羟基苯并呋喃}及其异构体莫拉辛 C（2-[3'，5'-二羟基-4'-(3-甲基-1-丁烯基）苯基]-6-羟基苯并呋喃}，使用与氧化还原相关的抗氧化测定法和非氧化还原抗氧化测定法进行了比较研究。在与氧化还原相关的抗氧化测定法中，莫拉辛 C 总是比 -moracin C 产生更高的 IC 值，包括•O₂抑制、Cu 还原能力、DPPH•抑制和 ABTS⁺•抑制测定法。在非氧化还原抗氧化测定法中，莫拉辛 C 和 -moracin C 经历了相似的自由基加成物形成（RAF），这在 HPLC-MS 光谱中 704 和 618 处的峰得到了证明。总之，莫拉辛 C 和 -moracin C 都可以作为 2-苯基苯并呋喃抗氧化剂；它们的抗氧化机制可能包括与氧化还原相关的 ET 和 H⁺转移，以及非氧化还原 RAF。在共轭位置的双键可以增强与氧化还原相关的抗氧化潜力，但几乎不影响 RAF 潜力。
