Woll Kellie A, Eckenhoff Roderic G
University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, United States.
University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, United States.
Methods Enzymol. 2018;602:177-187. doi: 10.1016/bs.mie.2018.01.007. Epub 2018 Mar 3.
General anesthetics are considered among the most significant advances in modern medicine; however, they are also some of the most dangerous commonly administered drugs. Despite this, the discovery of novel anesthetics has been slow, with few clinically used agents regardless of their nearly 200-year history. Xenopus laevis frogs have a long history as a model organism and provide a vital bridge between in vitro and preclinical mammalian assays. The provided protocols are efficient and cost-effective and therefore readily amendable for high-throughput evaluation of novel anesthetic ligands. By using the X. laevis bioassay, a researcher is capable of determining relative general anesthetic tolerance and/or cross-tolerance for candidate nonvolatile and/or volatile ligands.
全身麻醉剂被认为是现代医学中最重要的进展之一；然而，它们也是一些最危险的常用药物。尽管如此，新型麻醉剂的发现一直很缓慢，尽管其历史近200年，但临床上使用的药物却很少。非洲爪蟾作为一种模式生物有着悠久的历史，并且在体外和临床前哺乳动物试验之间提供了至关重要的桥梁。所提供的方案高效且具有成本效益，因此很容易修改以用于新型麻醉配体的高通量评估。通过使用非洲爪蟾生物测定法，研究人员能够确定候选非挥发性和/或挥发性配体的相对全身麻醉耐受性和/或交叉耐受性。
