Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
Semin Cell Dev Biol. 2019 Feb;86:92-101. doi: 10.1016/j.semcdb.2018.03.018. Epub 2018 Mar 28.
The essential bacteriophage T7-encoded single-stranded DNA binding protein is the nexus of T7 DNA metabolism. Multiple layers of macromolecular interactions mediate its function in replication, recombination, repair, and the maturation of viral genomes. In addition to binding ssDNA, the protein binds to DNA polymerase and DNA helicase, regulating their activities. The protein displays potent homologous DNA annealing activity, underscoring its role in recombination.
T7 噬菌体编码的必需单链 DNA 结合蛋白是 T7 DNA 代谢的枢纽。多种大分子相互作用层介导其在复制、重组、修复和病毒基因组成熟过程中的功能。除了结合 ssDNA 外，该蛋白还结合 DNA 聚合酶和 DNA 解旋酶，调节它们的活性。该蛋白显示出强大的同源 DNA 退火活性，强调了其在重组中的作用。
