1Department of Medical Statistics and Epidemiology, Guangzhou Key Laboratory of Environmental Pollution and Health Assessment, Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University, Room 715, 74 Zhongshan Road 2, Guangzhou, 510080 Guangdong China.
Department of Cancer Prevention Research, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.
Clin Epigenetics. 2018 Mar 22;10:39. doi: 10.1186/s13148-018-0472-5. eCollection 2018.
Fibroblast growth factor receptor 2 () gene encodes a protein of the fibroblast growth factor receptor family. gene expression is associated with the regulation of implantation process of placenta which plays a vital role in fetal growth. DNA methylation is widely known as a mechanism of fetal growth. However, it is unclear whether and how DNA methylation of gene in the placenta is associated with full-term low birth weight. This case-control study aims to explore the links between methylation in placenta and full-term low birth weight and to further examine the mediation effect of placental surface area on this association.
We conducted analyses for each of the five valid CpG sites at in 165 mother-baby pairs (86 FT-LBW vs. 79 FT-NBW) and found that per one standard deviation increase in the DNA methylation of CpG 11 at was associated with 1.64-fold higher risk of full-term low birth weight (OR = 1.64, 95% CI = [1.07, 2.52]) and 0.18 standard deviation decrease in placental surface area ( = - 0.18; standard error = 0.08, = 0.02). The mediation effect of placental surface area on the association between DNA methylation and full-term low birth weight was significant in girls (OR = 1.38, 95% CI = [1.05, 1.80]) but not in boys. The estimated mediation proportion was 48.38%.
Our findings suggested that placental surface area mediated the association between DNA methylation of in placenta and full-term low birth weight in a sex-specific manner. Our study supported the importance of placental epigenetic changes in placental development and fetal growth.
成纤维细胞生长因子受体 2() 基因编码成纤维细胞生长因子受体家族的蛋白质。基因表达与胎盘着床过程的调节有关，胎盘在胎儿生长中起着至关重要的作用。DNA 甲基化被广泛认为是胎儿生长的一种机制。然而，目前尚不清楚胎盘 基因的 DNA 甲基化是否以及如何与足月低出生体重有关。本病例对照研究旨在探讨胎盘 基因甲基化与足月低出生体重之间的联系，并进一步研究胎盘面积对此关联的中介作用。
我们对 165 对母婴（86 例 FT-LBW 与 79 例 FT-NBW）中的 5 个有效 CpG 位点在 上的进行了分析，发现 上 CpG 11 的 DNA 甲基化每增加一个标准差，足月低出生体重的风险就会增加 1.64 倍（OR=1.64，95%CI=[1.07, 2.52]），胎盘面积减少 0.18 个标准差（=−0.18；标准误=0.08，=0.02）。在女孩中，胎盘面积对 DNA 甲基化与足月低出生体重之间关联的中介作用是显著的（OR=1.38，95%CI=[1.05, 1.80]），但在男孩中则不显著。估计的中介比例为 48.38%。
我们的研究结果表明，胎盘面积以性别特异性的方式介导了胎盘 基因甲基化与足月低出生体重之间的关联。我们的研究支持了胎盘表观遗传变化在胎盘发育和胎儿生长中的重要性。
