Kang Jong Soon, Yoon Yeo Dae, Lee Myeong Youl, Lee Chang Woo, Lee Suk Jong, Han Sang-Bae
Korea Research Institute of Bioscience and Biotechnology, Cheongju, Chungbuk, Republic of Korea.
College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
Methods Mol Biol. 2018;1765:299-305. doi: 10.1007/978-1-4939-7765-9_19.
Cell-based immunotherapy for cancer is emerging as an attractive alternative to conventional small-molecule or antibody-based treatment. Due to the characteristics of cell-based therapy, validation of test materials before in vivo administration is required. Here we describe general validation steps for preclinical evaluation of cell-based immunotherapy. We also describe a xenograft model of human colorectal cancer. This model can be used for applied to preclinical evaluation of various cell-based therapy regimens for colorectal cancer treatment.
基于细胞的癌症免疫疗法正在成为传统小分子或抗体疗法颇具吸引力的替代方案。由于基于细胞疗法的特性，在体内给药前需要对测试材料进行验证。在此，我们描述了基于细胞的免疫疗法临床前评估的一般验证步骤。我们还描述了一种人类结直肠癌异种移植模型。该模型可用于对各种用于结直肠癌治疗的基于细胞的治疗方案进行临床前评估。
