Chollet-Martin S, Lavigne A, Gougerot-Pocidalo M A
INSERM U. 294, CHU Xavier Bichat, Paris.
Ann Biol Clin (Paris). 1987;45(3):304-7.
This study analyzes a method of indirect marking of T lymphocytes subpopulations using a 2nd antibody coupled to colloidal gold particles (Immunogold). There is an excellent correlation between this technique and the classical technique of indirect immunofluorescence. Immunogold enables an easy and rapid count of lymphocytes CD4+ and CD8+ during infections secondary to HIV virus (LAV/HTLV III). The permanent nature of the preparations and the cytological control make this method an interesting tool for the diagnosis and follow-up of patients suffering from AIDS, ARC or only asymptomatic seropositive individuals.
本研究分析了一种使用与胶体金颗粒偶联的第二抗体（免疫金）间接标记T淋巴细胞亚群的方法。该技术与间接免疫荧光经典技术之间存在极好的相关性。免疫金能够在HIV病毒（LAV/HTLV III）继发感染期间轻松快速地计数CD4+和CD8+淋巴细胞。制剂的永久性和细胞学对照使该方法成为诊断和随访艾滋病、艾滋病相关综合征患者或仅无症状血清阳性个体的有趣工具。
