Bird S J, Forster S, Lloyd J B
Department of Biological Sciences, University of Keele, Staffordshire, U.K.
Biochem J. 1987 Aug 1;245(3):929-31. doi: 10.1042/bj2450929.
A conflict exists in the literature concerning the mode of translocation of D-glucose and D-ribose across the lysosome membrane. The more rapid net uptake of ribose, when measured by the osmotic-protection technique, has been attributed either to its smaller size and lower hydrogen-bonding capacity, or to a lower affinity for a transport system shared by both sugars. The latency of acid beta-hexosaminidase in isolated rat liver lysosomes was measured after preincubation for periods up to 1 h in various solutions containing glucose and/or ribose, and in some cases sucrose. After confirmation of the superior osmotic protection afforded by glucose (than by ribose), it was shown that a solution 0.125 M in both glucose and ribose provided protection intermediate between that given by 0.25 M-glucose and that given by 0.25 M-ribose. This result is inconsistent with the common-carrier hypothesis.
关于D-葡萄糖和D-核糖跨溶酶体膜转运的模式，文献中存在争议。用渗透保护技术测量时，核糖的净摄取速度更快，这要么归因于其较小的尺寸和较低的氢键结合能力，要么归因于对两种糖共有的转运系统的较低亲和力。在含有葡萄糖和/或核糖以及某些情况下蔗糖的各种溶液中预孵育长达1小时后，测量分离的大鼠肝脏溶酶体中酸性β-己糖胺酶的潜伏时间。在证实葡萄糖提供的渗透保护优于核糖后，结果表明，葡萄糖和核糖浓度均为0.125M的溶液提供的保护介于0.25M葡萄糖和0.25M核糖所提供的保护之间。这一结果与共同载体假说不一致。
