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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1969 Dec;115(4):703-7. doi: 10.1042/bj1150703.

The latency of nitrocatechol sulphatase activity was measured in rat liver lysosomes before and after preincubation in 0.25m solutions of 25 different carbohydrates. 2. Preincubation in disaccharides, hexitols, gluconate, glucuronate or lactate gave little or no rise in ;free' sulphatase activity, indicating that these compounds do not easily penetrate the lysosomal membrane, but incubation in monosaccharides or the lower glycitols caused a progressive loss of latency. 3. Rates of increase in ;free' activity were taken as an indication of rates of solute penetration into lysosomes and were correlated with the structure and molecular weight of each sugar. 4. Additional evidence for non-penetration of maltose was obtained by demonstrating that the latency of lysosomal alpha-glucosidase is independent of substrate concentration employed. 5. The results are discussed in the light of published data on the latency of lysosomal enzymes.

在25种不同碳水化合物的0.25m溶液中预孵育前后，测定大鼠肝脏溶酶体中硝基儿茶酚硫酸酯酶活性的潜伏期。2. 在二糖、己糖醇、葡萄糖酸盐、葡萄糖醛酸盐或乳酸盐中预孵育，“游离”硫酸酯酶活性几乎没有增加或没有增加，这表明这些化合物不容易穿透溶酶体膜，但在单糖或低级糖醇中孵育会导致潜伏期逐渐丧失。3. “游离”活性的增加速率被用作溶质渗透到溶酶体中的速率指标，并与每种糖的结构和分子量相关。4. 通过证明溶酶体α-葡萄糖苷酶的潜伏期与所用底物浓度无关，获得了麦芽糖不渗透的额外证据。5. 根据已发表的关于溶酶体酶潜伏期的数据对结果进行了讨论。

Biochem J. 1969 Dec;115(4):703-7. doi: 10.1042/bj1150703.

The latency of nitrocatechol sulphatase activity was measured in rat liver lysosomes before and after preincubation in 0.25m solutions of 25 different carbohydrates. 2. Preincubation in disaccharides, hexitols, gluconate, glucuronate or lactate gave little or no rise in ;free' sulphatase activity, indicating that these compounds do not easily penetrate the lysosomal membrane, but incubation in monosaccharides or the lower glycitols caused a progressive loss of latency. 3. Rates of increase in ;free' activity were taken as an indication of rates of solute penetration into lysosomes and were correlated with the structure and molecular weight of each sugar. 4. Additional evidence for non-penetration of maltose was obtained by demonstrating that the latency of lysosomal alpha-glucosidase is independent of substrate concentration employed. 5. The results are discussed in the light of published data on the latency of lysosomal enzymes.

在25种不同碳水化合物的0.25m溶液中预孵育前后，测定大鼠肝脏溶酶体中硝基儿茶酚硫酸酯酶活性的潜伏期。2. 在二糖、己糖醇、葡萄糖酸盐、葡萄糖醛酸盐或乳酸盐中预孵育，“游离”硫酸酯酶活性几乎没有增加或没有增加，这表明这些化合物不容易穿透溶酶体膜，但在单糖或低级糖醇中孵育会导致潜伏期逐渐丧失。3. “游离”活性的增加速率被用作溶质渗透到溶酶体中的速率指标，并与每种糖的结构和分子量相关。4. 通过证明溶酶体α-葡萄糖苷酶的潜伏期与所用底物浓度无关，获得了麦芽糖不渗透的额外证据。5. 根据已发表的关于溶酶体酶潜伏期的数据对结果进行了讨论。