School of Physical Science, Jawaharlal Nehru University, New Delhi, India.
Bioelectronics, ICS8/PGI8, Forschungszentrum Juelich, Juelich, Germany.
Int J Nanomedicine. 2018 Mar 15;13(T-NANO 2014 Abstracts):55-58. doi: 10.2147/IJN.S125005. eCollection 2018.
Artificial lipid bilayer on solid substrate plays an important role as an interface between nanotechnology and biology. In this study, grid structures were patterned on Au-Nb-glass substrate and artificial bilayer was prepared on these structures. The fluidity was checked using fluorescence recovery after photobleaching (FRAP), and neuronal adhesion was monitored on such structure using EphrinA5-tethered lipid bilayer. EphrinA5 is a ligand that binds to the Eph receptors of rat cortical neurons and influences cellular adhesion. Our result elucidated that influence of these nanopatterned protein-tethered lipid bilayer on cellular guidance and signaling can address many underlying mechanisms of cellular functioning and help us to understand and differentiate the signaling procedure in cancer and neurodegenerative diseases.
固体基底上的人工脂质双层在纳米技术和生物学之间的界面中起着重要作用。在这项研究中，网格结构被图案化在 Au-Nb-玻璃基底上，并在这些结构上制备了人工双层。使用光漂白后荧光恢复（FRAP）检查了流动性，并使用 EphrinA5 键合脂质双层监测了这种结构上的神经元粘附。EphrinA5 是一种配体，它与大鼠皮质神经元的 Eph 受体结合并影响细胞粘附。我们的结果表明，这些纳米图案化的蛋白键合脂质双层对细胞导向和信号的影响可以解决细胞功能的许多潜在机制，并帮助我们理解和区分癌症和神经退行性疾病中的信号传递过程。
