Institute of Complex Systems ICS-8, Bioelectronics, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany.
Nanoscale. 2018 Mar 15;10(11):5295-5301. doi: 10.1039/c7nr08520f.
Biomimetic membranes create opportunities for various applications, including the possibility of replacing interacting cells in a cell-cell contact. Here we have fractionated synthetic membranes using metal nano-grid structures where EphrinA5 (EA5), a neuronal adhesion promoter, was anchored via its Fc domain (immunoglobulin G (IgG)-domain). FRAP experiments were performed to check the confinement of the synthetic membrane within these nano-structures. Rat cortical primary neurons were cultured and live cell imaging techniques were used to monitor the neuronal interaction with these fractionated synthetic membranes. Computational imaging analysis of the corresponding images elucidated interesting details of the cellular behavior. The phenotypic cellular response on these nano-membrane fractions was found to be similar to that on non-fractionated synthetic membranes indicating that although the number of focal adhesion points was low (due to the reduced EA5 number) in the nano-sized membrane patches perhaps some other factors like metal grid boundaries might be playing a role in rendering the similarity.
仿生膜为各种应用创造了机会，包括替代细胞-细胞接触中相互作用的细胞的可能性。在这里，我们使用金属纳米网格结构对合成膜进行了分级，其中 EphrinA5（EA5）通过其 Fc 结构域（免疫球蛋白 G（IgG）-结构域）锚定在膜上。进行 FRAP 实验以检查合成膜在这些纳米结构内的限制。培养大鼠皮质原代神经元，并使用活细胞成像技术监测神经元与这些分级合成膜的相互作用。对相应图像的计算成像分析阐明了细胞行为的有趣细节。在这些纳米膜片上的表型细胞反应与非分级合成膜上的反应相似，表明尽管纳米尺寸的膜片中的粘着斑点数较少（由于 EA5 数量减少），但其他一些因素，如金属网格边界，可能在产生相似性方面发挥作用。
