通过同位素比率质谱法测定生物体液中的氘水平。
Determination of deuterium level in biological fluids by isotope ratio mass spectrometry.
作者信息
Penman A D, Wright I A
机构信息
Hill Farming Research Organisation, Bush Estate, Scotland, UK.
出版信息
Biomed Environ Mass Spectrom. 1987 Jul;14(7):339-42. doi: 10.1002/bms.1200140708.
Water is produced by vacuum sublimation of biological fluids directly into reaction vessels in preparation for reduction using zinc. Following heating to 450 degrees C the hydrogen produced is admitted directly to the mass spectrometer. The standard derivation, sigma 10 of analysis of water samples was 1.08-1.23% while for blood plasma sigma 6 = 2.02-2.62%. The method allows the determination of very low levels of deuterium in biological fluids, thus allowing very small doses of deuterium oxide to be used in studies of body water in animals and possibly humans.
通过将生物流体真空升华直接导入反应容器来制备水，以便用锌进行还原。加热至450摄氏度后，产生的氢气直接导入质谱仪。水样分析的标准偏差σ10为1.08 - 1.23%，而血浆的σ6为2.02 - 2.62%。该方法能够测定生物流体中极低水平的氘，从而在动物乃至可能在人体的体内水研究中使用极少量的氧化氘。
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