MOE Key Laboratory of Environment and Genes Related to Diseases, School of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an, China.
MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China.
Front Immunol. 2018 Mar 12;9:511. doi: 10.3389/fimmu.2018.00511. eCollection 2018.
Native C-reactive protein (nCRP) is a non-specific marker of inflammation being claimed as a bystander in several chronic disorders. Accumulating evidence indicates that nCRP dissociates to and acts primarily as the monomeric conformation (mCRP) at inflammatory loci. This suggests that mCRP may be a superior disease marker with improved specificity and clear causality to the underlying pathogenesis. However, the lack of a feasible assay to quantify mCRP in clinical samples precludes the assessment of that suggestion. Here we report the development of a sandwich ELISA assay for quantification of plasma mCRP using commercially available reagents. Our assay is reproducible and highly conformation-specific showing a reliable detection limit of 1 ng/mL. We further show that mCRP appears to be a better marker than nCRP in several skin-related autoimmune disorders. This assay thus provides a useful tool to examine the clinical significance and utility of mCRP.
天然 C 反应蛋白 (nCRP) 是一种非特异性炎症标志物，被认为是几种慢性疾病的旁观者。越来越多的证据表明，nCRP 分离并主要作为单体构象 (mCRP) 在炎症部位发挥作用。这表明 mCRP 可能是一种更好的疾病标志物，具有更高的特异性和对潜在发病机制的明确因果关系。然而，缺乏一种可行的测定方法来定量检测临床样本中的 mCRP，这限制了对该假设的评估。在这里，我们报告了一种使用市售试剂定量检测血浆 mCRP 的夹心 ELISA 测定方法的开发。我们的测定方法具有可重复性和高度构象特异性，显示出可靠的检测限为 1ng/mL。我们还表明，mCRP 在几种与皮肤相关的自身免疫性疾病中似乎是比 nCRP 更好的标志物。因此，该测定方法为研究 mCRP 的临床意义和实用性提供了有用的工具。
