Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Mikrochim Acta. 2018 Jan 19;185(2):121. doi: 10.1007/s00604-017-2654-9.
Mesoporous carbon foam (MCF) was prepared by via the Pechini method which is facile and template-free. The MCF was characterized by field emission scanning electron microscopy, transmission electron microscopy, X-ray diffraction and BET surface area analysis. Afterwards, the MCF was dispersed in the natural hydrogel salep to give a composite. Finally, myoglobin was immobilized on the composite and then placed on a glassy carbon electrode (GCE). The modified GCE gives a distinct quasi-reversible redox peak during electroreduction of hydrogen perxide (HO). The estimated electron transfer coefficient (α) and the heterogeneous electron transfer rate constant (ks) for redox process of Mb are 0.54 and 2.25 s, respectively. The sensor, best operated at -0.2 V (vs. Ag/AgCl), responds to HO in the 1.0 to 80 μM HO concentration range, with a 180 nM limit of detection (at S/N ratio of 3). The technique was applied to the determination of HO in spiked fetal bovine serum samples. Graphical abstract Mesoporous carbon foam (MCF) synthesis, dispersion in Salep solution, preparing the Salep-MCF composite (S-MCF), immobilizing the Mb at S-MCF and preparing Mb/S-MCF composite, Casting the Mb/S-MCF on electrode surface to prepare Mb/S-MCF/GCE and electrochemical behavior of biosensor.
介孔碳泡沫（MCF）通过 Pechini 法制备，该方法简便且无需模板。通过场发射扫描电子显微镜、透射电子显微镜、X 射线衍射和 BET 比表面积分析对 MCF 进行了表征。然后，将 MCF 分散在天然水凝胶 Salep 中得到复合材料。最后，将肌红蛋白固定在复合材料上，然后将其置于玻碳电极（GCE）上。在过氧化物（HO）的电还原过程中，修饰后的 GCE 给出了明显的准可逆氧化还原峰。肌红蛋白氧化还原过程的估计电子转移系数（α）和非均相电子转移速率常数（ks）分别为 0.54 和 2.25 s。该传感器在-0.2 V（相对于 Ag/AgCl）下最佳运行，在 1.0 至 80 μM 的 HO 浓度范围内对 HO 有响应，检测限为 180 nM（信噪比为 3）。该技术已应用于测定胎牛血清样品中 HO 的含量。
