Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Department of Organic Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Mikrochim Acta. 2018 Feb 17;185(3):182. doi: 10.1007/s00604-017-2599-z.
The authors show that copper nanoclusters capped with tannic acid (TA-CuNCs) are viable fluorescent probes for the determination of the activity of pyrophosphatase (PPase). The fluorescence of the TA-CuNCs is quenched by Fe(III) but restored on subsequant addition of pyrophosphate (PPi). If, however, PPi is split by PPase into two phosphate ions, the fluorescence of the TA-CuNCs is quenched again. Under optimized conditions, fluorescence intensity linearly correlates with the activity of PPase in the range from 0.50 to 18.0 U L with a detection limit of 0.19 U L. The method was employed to the determination of PPase in spiked human serum samples and gave satisfactory results. Graphical abstract Schematic illustration of fluorometric assay for PPase activity. It is based on the finding that pyrophosphate (PPi) can restore the Fe-quenched TA-CuNC fluorescence. PPi is formed by enzymatic action of PPasei.
作者表明，单宁酸（TA）包覆的铜纳米簇（TA-CuNCs）是测定焦磷酸酶（PPase）活性的可行荧光探针。TA-CuNCs 的荧光被 Fe(III)猝灭，但随后加入焦磷酸盐（PPi）后会恢复。然而，如果 PPi 被 PPase 裂分为两个磷酸离子，TA-CuNCs 的荧光将再次猝灭。在优化条件下，荧光强度与 PPase 的活性在 0.50 至 18.0 U L 的范围内呈线性相关，检测限为 0.19 U L。该方法用于测定加标人血清样品中的 PPase，结果令人满意。
