Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou, 450001, China.
Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, School of Materials and Chemical Engineering, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou, 450001, China.
Mikrochim Acta. 2018 Feb 19;185(3):186. doi: 10.1007/s00604-018-2729-2.
The paper describes a one-step synthetic method to chemically reduce cupric sulfate by ascorbic acid in the presence of DNA strands to directly produce Cu O@DNA spheres. The DNA strands act as template to assist the preparation of Cu O, and also are capable of specifically binding Pb(II) ions. The Cu O@DNA spheres possess high specific surface area and strong bioaffinity. They can be directly employed as platform for detecting Pb sensitively. Electrochemical impedance spectroscopy data showed that the assay exhibits high sensitivity and a wide linear analytical range that extends from 0.1 to 100 nM, and the detection limit is 6.8 pM at a signal-to-noise ratio of 3. The assay is selective, acceptably reproducible, stable, and well feasible for the detection of Pb in blood serum. Graphical abstract Schematic presentation of the preparation of DNA-templated Cu O spheres (Cu O@DNA) for use in electrochemical detection of Pb. The assay exhibits detection limit of 6.8 pM, high selectivity, acceptable reproducibility, stability, and good applicability for Pb detection.
本文描述了一种一步合成方法，即在 DNA 链的存在下，通过抗坏血酸将硫酸铜化学还原为直接产生 CuO@DNA 球的 CuO@DNA 球。DNA 链作为模板辅助 CuO 的制备，并且还能够特异性结合 Pb(II)离子。CuO@DNA 球具有高比表面积和强生物亲和力。它们可以直接用作灵敏检测 Pb 的平台。电化学阻抗谱数据表明，该测定法具有高灵敏度和宽线性分析范围，从 0.1 到 100 nM，检测限为 6.8 pM，信噪比为 3。该测定法具有选择性、可接受的重现性、稳定性，并且非常适用于血清中 Pb 的检测。 图表摘要 用于电化学检测 Pb 的 DNA 模板 CuO 球 (CuO@DNA) 的制备示意图。该测定法的检测限为 6.8 pM，具有高选择性、可接受的重现性、稳定性和良好的适用性，可用于 Pb 检测。
