Donati Manuela, Laroucau Karine, Guerrini Alessandro, Balboni Andrea, Salvatore Daniela, Catelli Elena, Lupini Caterina, Levi Aurora, Di Francesco Antonietta
1 Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna , Bologna, Italy .
2 Bacterial Zoonoses Unit, Animal Health Laboratory, University Paris-Est , Anses, Maisons-Alfort, France .
Vector Borne Zoonotic Dis. 2018 Apr;18(4):222-225. doi: 10.1089/vbz.2017.2211. Epub 2018 Mar 8.
Until recently, Chlamydia psittaci was considered to be the only etiological agent of avian chlamydiosis, but two new avian species, Chlamydia gallinacea and Chlamydia avium, have recently been described in poultry and pigeons or psittacine birds, respectively. The aim of this study was to explore the occurrence of C. psittaci and C. gallinacea in backyard chickens in Italy. Cloacal swabs were taken from 160 asymptomatic chickens reared in 16 backyard farms. Samples were tested for C. psittaci and C. gallinacea by specific real-time polymerase chain reaction assays, with 24 (15%) of the 160 chickens resulting positive for C. gallinacea. To attempt chlamydial isolation, new samples were obtained from two farms harboring a high prevalence (60% and 70%, respectively) of C. gallinacea-positive chickens. In total, eight C. gallinacea and one C. psittaci isolates were successfully recovered from 13 chickens. C. gallinacea was confirmed to be the endemic chlamydial species in chickens, with a high ompA intraspecies diversity. The presence of viable C. psittaci and C. gallinacea demonstrated by isolation from chickens in backyard farms poses a potential public health problem.
直到最近，鹦鹉热衣原体仍被认为是禽衣原体病的唯一病原体，但最近分别在家禽和鸽子或鹦鹉中发现了两种新的禽类衣原体，即鸡衣原体和鸟衣原体。本研究的目的是探究意大利散养鸡中鹦鹉热衣原体和鸡衣原体的感染情况。从16个后院农场饲养的160只无症状鸡中采集泄殖腔拭子。通过特异性实时聚合酶链反应检测样本中的鹦鹉热衣原体和鸡衣原体，160只鸡中有24只（15%）鸡衣原体检测呈阳性。为尝试衣原体分离，从两个鸡衣原体阳性鸡患病率较高（分别为60%和70%）的农场获取了新样本。总共从13只鸡中成功分离出8株鸡衣原体和1株鹦鹉热衣原体。鸡衣原体被确认为鸡中的地方性衣原体物种，其ompA种内多样性较高。从后院农场的鸡中分离出活的鹦鹉热衣原体和鸡衣原体，这构成了潜在的公共卫生问题。
