Cai Pumo, Hong Jingfang, Wang Cong, Yang Yanchuan, Zhang Qiwen, Ji Qinge, Chen Jiahua
Institute of Beneficial Insects, Plant Protection College, Fujian Agriculture and Forestry University, Fuzhou, China.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou, China.
J Econ Entomol. 2018 May 28;111(3):1157-1164. doi: 10.1093/jee/toy032.
This study explored the potential for Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) larvae hatched from irradiated eggs as hosts for Diachasmimorpha longicaudata (Ashmead) (Hymenoptera: Braconidae). B. dorsalis eggs of three different ages (12-, 24-, and 36-h old) were analyzed for hatchability, pupation rate, pupal weight, emergence rate, and sex ratio after exposure to different doses of radiation (5 and 10 Gy) at different dose rates (1 and 6 Gy/min). For the eggs of different ages exposed to radiation, only the hatchability and pupal weight of 36-h-old eggs exposed to the dose rate of 1 Gy/min were not affected; therefore, 6 Gy/min was not suitable for irradiating eggs. The viability of the parents and progenies of D. longicaudata when the parents were reared from 36-h-old eggs irradiated at nine different doses (0, 5, 10, 15, 20, 25, 30, 35, and 40 Gy) under laboratory conditions were investigated. The emergence percentage, sex ratio, and longevity of parasitoids developed from irradiated eggs were similar to those reared from nonirradiated hosts. A significant increase in larva mortality was observed for the eggs irradiated at doses above 25 Gy, and no redundant adult flies emerged at doses above 15 Gy. Hence, for B. dorsalis eggs to be applied in the mass rearing of D. longicaudata, the age of 36 h and a dose of 20-25 Gy are the optimal parameters. The results reveal that hosts and parasitoids need not be separated, enabling a reduction in cost, labor, and time and resulting in an improved mass rearing procedure for D. longicaudata.
本研究探讨了经辐照卵孵化出的橘小实蝇（亨德尔）（双翅目：实蝇科）幼虫作为长尾潜蝇茧蜂（阿什米德）（膜翅目：茧蜂科）寄主的潜力。分析了三种不同龄期（12小时、24小时和36小时）的橘小实蝇卵在不同剂量率（1和6戈瑞/分钟）下暴露于不同剂量辐射（5和10戈瑞）后的孵化率、化蛹率、蛹重、羽化率和性别比。对于暴露于辐射的不同龄期的卵，仅暴露于1戈瑞/分钟剂量率的36小时龄卵的孵化率和蛹重未受影响；因此，6戈瑞/分钟不适合辐照卵。在实验室条件下，研究了长尾潜蝇茧蜂的亲本和子代在从九种不同剂量（0、5、10、15、20、25、30、35和40戈瑞）辐照的36小时龄卵饲养时的活力。从辐照卵发育而来拟寄生蜂的羽化率、性别比和寿命与从未辐照寄主饲养的相似。在剂量高于25戈瑞时观察到辐照卵的幼虫死亡率显著增加，且在剂量高于15戈瑞时没有多余的成虫羽化。因此，对于应用于长尾潜蝇茧蜂大规模饲养的橘小实蝇卵，36小时龄和20 - 25戈瑞的剂量是最佳参数。结果表明，寄主和拟寄生蜂无需分离，从而降低了成本、劳动力和时间，并改进了长尾潜蝇茧蜂的大规模饲养程序。
