School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor, Malaysia.
Malaysia Genome Institute, Jalan Bangi Lama, 43000, Kajang, Selangor, Malaysia.
Mar Environ Res. 2018 Jun;137:169-176. doi: 10.1016/j.marenvres.2018.03.007. Epub 2018 Mar 21.
Glaciozyma antarctica PI12, is a psychrophilic yeast isolated from Antarctic sea. In this work, Expressed Sequence Tags (EST) from cells exposed to three different temperatures; 15 °C, 0 °C and -12 °C were generated to identify genes associated with cold adaptation. A total of 5376 clones from each library were randomly picked and sequenced. Comparative analyses from the resulting ESTs in each condition identified several groups of genes required for cold adaptation. Additionally, 319 unique transcripts that encoded uncharacterised functions were identified in the -12 °C library and are currently unique to G. antarctica. Gene expression analysis using RT-qPCR revealed two of the unknown genes to be up-regulated at -12 °C compared to 0 °C and 15 °C. These findings further contribute to the collective knowledge into G. antarctica cold adaptation and as a resource for understanding the ecological and physiological tolerance of psychrophilic microbes in general.
南极冰酵母 PI12 是一种从南极海洋中分离出来的嗜冷酵母。在这项工作中，我们生成了暴露于三种不同温度（15°C、0°C 和-12°C）下的细胞的表达序列标签（EST），以鉴定与冷适应相关的基因。每个文库中总计随机挑选了 5376 个克隆进行测序。对每种条件下产生的 EST 的比较分析鉴定了适应寒冷所需的几组基因。此外，在-12°C 文库中还鉴定到 319 个编码未知功能的独特转录本，目前这些转录本仅存在于 G. antarctica 中。使用 RT-qPCR 的基因表达分析显示，与 0°C 和 15°C 相比，两个未知基因在-12°C 下上调。这些发现进一步为 G. antarctica 适应寒冷的集体知识做出了贡献，并为理解一般嗜冷微生物的生态和生理耐受性提供了资源。
