Satake Tomoyuki, Suetsugu Atsushi, Nakamura Miki, Hasegawa Kosuke, Kunisada Takahiro, Shimizu Masahito, Saji Shigetoyo, Moriwaki Hisataka, Hoffman Robert M
Gifu University Graduate School of Medicine, Gifu, Japan.
Gifu University Graduate School of Medicine, Gifu, Japan
Anticancer Res. 2018 Apr;38(4):1927-1935. doi: 10.21873/anticanres.12430.
BACKGROUND/AIM: The lethal characteristic of pancreatic cancer is metastasis which is recalcitrant to currently-used chemotherapy. Our aim was to understand metastasis at the cellular level. We previously reported that multi-nucleate cells or spindle cells were more prominent in pancreatic cancer metastasis than in the primary tumor. In the present report, we investigated four representative human pancreatic cell lines for differences in cell morphology between the primary tumor and various metastatic organ targets for each cell line.
Human pancreatic cancer cell lines AsPC-1, Panc-1, KP2 and KP3 were used. Pancreatic cancer cells were injected into spleen of nude mice resulting in experimental metastasis to various organs which were observed at the cellular level when the organs were placed into culture.
AsPC-1 and KP2 pancreatic cells formed many experimental liver metastases, in contrast to Panc-1 and KP3. Lung metastasis was only observed for AsPC-1. In the cultures established from the primary and metastatic tumors, multi-nucleate cells were found to be more prominent in the metastasis of the pancreatic cell lines with frequent metastasis, AsPC-1 and KP2. Spindle-like cells were observed prominently in AsPC-1 lung metastasis.
Human pancreatic cancer cell lines have differential metastatic characteristics with regard to target organs and cell-morphology changes. Multi-nucleate and spindle cells may play an important role in pancreatic cancer metastasis to the liver and lung, respectively.
背景/目的：胰腺癌的致命特征是转移，而这对目前使用的化疗具有抗性。我们的目的是在细胞水平上了解转移情况。我们之前报道过，多核细胞或梭形细胞在胰腺癌转移中比在原发性肿瘤中更为突出。在本报告中，我们研究了四种具有代表性的人胰腺癌细胞系，以了解每种细胞系原发性肿瘤与各种转移器官靶点之间细胞形态的差异。
使用人胰腺癌细胞系AsPC-1、Panc-1、KP2和KP3。将胰腺癌细胞注射到裸鼠脾脏中，导致实验性转移至各种器官，当将这些器官置于培养中时，可在细胞水平上进行观察。
与Panc-1和KP3相比，AsPC-1和KP2胰腺细胞形成了许多实验性肝转移灶。仅在AsPC-1中观察到肺转移。在从原发性和转移性肿瘤建立的培养物中，发现多核细胞在转移频繁的胰腺细胞系AsPC-1和KP2的转移中更为突出。在AsPC-1肺转移中明显观察到梭形细胞。
人胰腺癌细胞系在靶器官和细胞形态变化方面具有不同的转移特征。多核细胞和梭形细胞可能分别在胰腺癌转移至肝脏和肺中发挥重要作用。
