Gifu University Graduate School of Medicine, Gifu, Japan.
Gifu University Graduate School of Medicine, Gifu, Japan;
Anticancer Res. 2021 Jun;41(6):2829-2834. doi: 10.21873/anticanres.15063.
BACKGROUND/AIM: Cancer-derived exosomes play an important role in metastasis. In the present study, we determined whether exosome transfer between cancer cells is associated with metastasis in a mouse model.
AsPC-1 human pancreatic-cancer cells expressing red fluorescent protein (RFP) and AsPC-1 human pancreatic-cancer cells transduced by exosome-specific pCT-CD63-green fluorescent protein (GFP), were co-injected into the spleen of nude mice.
Both pancreatic-cancer cell lines grew in the spleen and metastasized to the liver, peritoneum, and lungs, as shown by color-coded imaging. The ratio of GFP-expressing exosomes incorporated in RFP-labeled AsPC-1 cells was statistically-significantly higher in the liver, lung, and peritoneal metastases than in the spleen.
Exosome transfer between cancer cells is associated with metastasis. Exosome transfer may play a role in increasing the metastatic capability of the recipient cells.
背景/目的：癌细胞衍生的外泌体在转移中发挥重要作用。在本研究中，我们确定了癌细胞之间的外泌体转移是否与小鼠模型中的转移有关。
表达红色荧光蛋白（RFP）的 AsPC-1 人胰腺癌细胞和经外泌体特异性 pCT-CD63-绿色荧光蛋白（GFP）转导的 AsPC-1 人胰腺癌细胞被共同注射到裸鼠的脾脏中。
两种胰腺癌细胞系均在脾脏中生长，并转移到肝脏、腹膜和肺部，通过彩色编码成像显示。在肝、肺和腹膜转移中，GFP 表达的外泌体整合到 RFP 标记的 AsPC-1 细胞中的比例明显高于脾脏。
癌细胞之间的外泌体转移与转移有关。外泌体转移可能在增加受体细胞的转移能力方面发挥作用。
