El-Deeb Amal M, Fansa Hoda A, Wahba Omneya M
Department of Oral Pathology, Faculty of Dentistry, Tanta University, Tanta, Egypt.
Department of Oral Biology, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
Int J Health Sci (Qassim). 2018 Mar-Apr;12(2):22-25.
The present work evaluated histologically and immunohistochemically the expression of leptin during healing of the incisional oral mucosal wound in diabetic rats as compared to healthy rats.
Twenty-four adult male Sprague-Dawley rats weighing on average 150-200 g were allocated equally into two groups: Group I (control) and Group II (diabetic). Diabetes was induced by a single intraperitoneal injection of streptozotocin dissolved in distilled water. Each animal received experimental incision in buccal mucosa and sutured, and the specimens were collected from the buccal mucosa of each animal at intervals of 7, 14, and 21 days and routinely processed for H and E and immunohistochemical staining for leptin. All measurement data were calculated as a mean ± standard deviation.
Leptin expression was observed in the epithelium and the vascular endothelial cells in both groups. In both the control and diabetic groups, the expression of leptin was significantly increased with time, and there was an extreme highly significant increase in the control group than in diabetic group after 7, 14, and 21 days ( = 0.000).
The results of the present study suggested that leptin may promote wound healing in rat's normal oral mucosa more than in diabetic. Further studies are needed to clarify the exact molecular mechanisms of leptin's effects on wound healing and to determine the usefulness of leptin as a treatment to promote wound healing in the oral mucosa in diabetic and non-diabetic patients.
与健康大鼠相比，本研究通过组织学和免疫组织化学方法评估了糖尿病大鼠口腔黏膜切口愈合过程中瘦素的表达情况。
将24只平均体重为150 - 200克的成年雄性Sprague-Dawley大鼠平均分为两组：第一组（对照组）和第二组（糖尿病组）。通过腹腔注射溶解于蒸馏水中的链脲佐菌素诱导糖尿病。每只动物在颊黏膜处进行实验性切口并缝合，在第7、14和21天从每只动物的颊黏膜采集标本，常规进行苏木精-伊红染色及瘦素免疫组织化学染色。所有测量数据均以平均值±标准差计算。
两组的上皮细胞和血管内皮细胞中均观察到瘦素表达。在对照组和糖尿病组中，瘦素表达均随时间显著增加，且在第7、14和21天后，对照组的增加幅度极显著高于糖尿病组（P = 0.000）。
本研究结果表明，瘦素对大鼠正常口腔黏膜伤口愈合的促进作用可能大于糖尿病状态下的口腔黏膜。需要进一步研究以阐明瘦素影响伤口愈合的确切分子机制，并确定瘦素作为促进糖尿病和非糖尿病患者口腔黏膜伤口愈合治疗方法的有效性。
