Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam 50612, Republic of Korea.
Korean Medical Science Research Center for Healthy Aging, Pusan National University, Yangsan, Gyeongnam 50612, Republic of Korea.
Oxid Med Cell Longev. 2018 Feb 11;2018:2479602. doi: 10.1155/2018/2479602. eCollection 2018.
Although stroke is among the leading causes of death and long-term disability, there are few effective treatments for limiting the severity of neurological sequelae. We evaluated the effects of 29 medicinal herbs listed in the chapter of the 17th century Korean medical text on stroke symptoms in a mouse model of cerebral ischemia.
Focal cerebral ischemia was induced via photothrombosis. Infarct volume, brain edema, and neurological deficits were evaluated. Immunofluorescence staining for tight junction proteins and aquaporin 4 (AQP4) was performed following ischemic injury.
Based on our initial findings, we examined the effects of two prescriptions in which the candidate herbs comprised more than 60% of the total formula: Shuanghe-tang and Zengsunsiwu-tang. Pretreatment with Shuanghe-tang significantly reduced infarct volume, decreased blood-brain barrier (BBB) breakdown, attenuated edema, and improved neurological and motor functions in a dose-dependent manner (30, 100, and 300 mg/kg), while no such effects were observed in mice pretreated with Zengsunsiwu-tang. Immunohistochemical analysis revealed significant increases in ipsilateral occludin and zonula occludens 1 (ZO-1) expression in Shuanghe-tang-pretreated mice, as well as increased AQP4 immunofluorescence.
These results indicate that Shuanghe-tang may protect against brain injury and promote recovery of neurological function following ischemia.
尽管中风是导致死亡和长期残疾的主要原因之一，但目前针对限制神经后遗症严重程度的有效治疗方法却寥寥无几。我们评估了 17 世纪朝鲜医学文本中列出的 29 种草药对大脑局部缺血模型中中风症状的影响。
通过光血栓形成诱导局灶性脑缺血。评估梗死体积、脑水肿和神经功能缺损。缺血损伤后进行紧密连接蛋白和水通道蛋白 4（AQP4）的免疫荧光染色。
根据我们的初步发现，我们研究了两种方剂的效果，其中候选草药占总配方的 60%以上：双和汤和增损四物汤。双和汤预处理可显著降低梗死体积，减少血脑屏障（BBB）破坏，减轻水肿，改善神经和运动功能，呈剂量依赖性（30、100 和 300mg/kg），而增损四物汤预处理的小鼠则没有这种效果。免疫组织化学分析显示，双和汤预处理小鼠同侧 occludin 和 zonula occludens 1（ZO-1）表达显著增加，AQP4 免疫荧光也增加。
这些结果表明，双和汤可能对脑损伤具有保护作用，并能促进缺血后神经功能的恢复。
