Hassan Md Sazzad, von Holzen Urs
Department of Surgery, Indiana University School of Medicine, South Bend, IN, USA.
Harper Cancer Research Institute, South Bend, IN, USA.
Methods Mol Biol. 2018;1756:151-164. doi: 10.1007/978-1-4939-7734-5_14.
Researchers often use murine models of esophageal cancer to evaluate novel therapies prior to clinical protocol treatment. Subcutaneous xenograft models are often used for testing the efficacy of anticancer agents in many cancers including esophageal adenocarcinoma. However, mice subcutaneous esophageal adenocarcinoma models only represent local tumor growth and do not provide any information about a survival benefit for a particular anticancer regimen, which is very crucial for experimental treatment efficacy. In addition, anticancer agents may well inhibit subcutaneous tumor growth without effecting overall animal survival. Herein, we describe a peritoneal dissemination mouse xenograft model for survival outcome analysis with intraperitoneal injection of human esophageal adenocarcinoma cell lines.
研究人员常在临床方案治疗前使用食管癌小鼠模型来评估新疗法。皮下异种移植模型常用于测试抗癌药物在包括食管腺癌在内的多种癌症中的疗效。然而，小鼠皮下食管腺癌模型仅代表局部肿瘤生长，无法提供关于特定抗癌方案生存获益的任何信息，而这对于实验性治疗效果至关重要。此外，抗癌药物可能很好地抑制皮下肿瘤生长而不影响动物总体生存。在此，我们描述一种通过腹腔注射人食管腺癌细胞系用于生存结果分析的腹膜播散小鼠异种移植模型。
