Islam Farhadul, Gopalan Vinod, Lam Alfred K
Cancer Molecular Pathology of School of Medicine, Griffith University, Gold Coast, Australia.
Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh.
Methods Mol Biol. 2018;1756:165-176. doi: 10.1007/978-1-4939-7734-5_15.
Cancer stem cells (CSCs) are a subpopulation of cancer cells that have the ability to self-renew and to generate differentiated cells of various lineages. Due to their specific morphological and biological features, they are often resistant to therapy and in turn lead to metastasis and cancer recurrence. Because of their crucial roles in carcinogenesis and patient prognosis, identification and isolation of CSCs have become an important part of improved cancer management regime. Isolation, characterization, and development of targeted therapy against CSCs have potential efficacy in treating esophageal cancer. In addition, CSCs can act as a predictive tool for chemoradiotherapy response in esophageal adenocarcinoma. Different methods including functional assays, cell sorting using various intracellular, and cell surface markers and xenotransplantation techniques are used for the identification and separation of CSCs in different cancers. None of these methods solely can guarantee complete isolation of CSC population, thus a combination of methods could be used for reliable detection and isolation of CSCs. Here, we describe the identification and isolation of CSCs from esophageal adenocarcinoma cells by cell sorting after Hoechst 33342 staining followed by in vitro functional assays, and in vivo xenograft techniques.
癌症干细胞（CSCs）是癌细胞的一个亚群，具有自我更新能力，并能产生各种谱系的分化细胞。由于其特定的形态和生物学特征，它们通常对治疗有抗性，进而导致转移和癌症复发。鉴于它们在致癌作用和患者预后方面的关键作用，癌症干细胞的识别和分离已成为改善癌症管理方案的重要组成部分。针对癌症干细胞的分离、表征和靶向治疗的开发在治疗食管癌方面具有潜在疗效。此外，癌症干细胞可作为食管腺癌放化疗反应的预测工具。包括功能测定、使用各种细胞内和细胞表面标志物的细胞分选以及异种移植技术在内的不同方法，被用于识别和分离不同癌症中的癌症干细胞。这些方法单独一种都不能保证完全分离癌症干细胞群体，因此可以联合使用多种方法来可靠地检测和分离癌症干细胞。在此，我们描述了通过Hoechst 33342染色后进行细胞分选，随后进行体外功能测定和体内异种移植技术，从食管腺癌细胞中识别和分离癌症干细胞的方法。
