Pan Xuan-Xuan, Song Fen-Yun, Lin Xiu-Lian, Li Hua
Guangdong Pharmaceutical University， Guangzhou 510006， China.
Guangdong Institute for Drug Control， Guangzhou 510180， China.
Zhongguo Zhong Yao Za Zhi. 2018 Feb;43(3):527-531. doi: 10.19540/j.cnki.cjcmm.20171016.004.
To analyze the metabolites of Chenxiang Huaqi pill in rats by using ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS). The separation was performed on Phenomenex Kinetex C₁₈ column, with the acetonitrile -0.1% formic acid as the mobile phase for gradient elution at a flow rate of 0.8 mL·min⁻¹. The data were collected by the positive ion mode of ESI source. The plasma and urine total ion chromatograms of the rats in blank group and treatment group were used to analyze the targeted ion chromatograms. The results showed that 24 compounds were detected in the plasma and urine, including 5 prototype components and 19 metabolites. The major metabolic pathways included hydration, glucuronidation, demethylation, hydrolysis, hydroxylation and sulfation. The method was rapid, simple and sensitive, and can be used to rapidly identify the metabolites of Chenxiang Huaqi pill that can be absorbed in rats, providing a reference for the study of the absorption and metabolism mechanism of Chenxiang Huaqi pill .
采用超高效液相色谱-四极杆-飞行时间质谱（UPLC-Q-TOF-MS）分析大鼠体内沉香化气丸的代谢产物。采用Phenomenex Kinetex C₁₈色谱柱进行分离，以乙腈-0.1%甲酸为流动相进行梯度洗脱，流速为0.8 mL·min⁻¹。采用电喷雾离子源（ESI）正离子模式采集数据。利用空白组和给药组大鼠的血浆和尿液总离子流色谱图分析目标离子色谱图。结果表明，在血浆和尿液中检测到24种化合物，包括5种原型成分和19种代谢产物。主要代谢途径包括水化、葡萄糖醛酸化、去甲基化、水解、羟基化和硫酸化。该方法快速、简便、灵敏，可用于快速鉴定大鼠体内可吸收的沉香化气丸代谢产物，为研究沉香化气丸吸收代谢机制提供参考。
