Chen Si-Ying, Xia Tao, Zheng Lin, Li Yue-Ting, Gong Zi-Peng, Huang Yong, Li Yong-Jun, Zhao Ping
Guizhou Provincial Key Laboratory of Pharmaceutics State Key Laboratory of Efficacy and Utilization of Medicinal Plants,Guizhou Medical University Guiyang 550004,China.
Guizhou Provincial Key Laboratory of Pharmaceutics State Key Laboratory of Efficacy and Utilization of Medicinal Plants,Guizhou Medical University Guiyang 550004,China School of Pharmacy,Guizhou Medical University Guiyang 550004,China.
Zhongguo Zhong Yao Za Zhi. 2019 May;44(10):2156-2162. doi: 10.19540/j.cnki.cjcmm.20190114.002.
Ultra performance liquid chromatography coupled with time-of-flight mass spectrometry( UPLC-Q-TOF-MS/MS) method was applied to analyze the prototypes and metabolites of the effective components of Polygonum orientale in SD rat serum and urine. The separation was performed on Agilent Eclipse Plus C_(18) column( 2. 1 mm×100 mm,1. 8 μm),with 0. 1% formic acid solution( A)-acetonitrile( B) as the mobile phase for gradient elution. Mass spectrometry data of biological samples were obtained under positive and negative electrospray ion mode. By comparing chromatogram differences between blank samples and drug treatment samples,prototype components and metabolites of the effective components of P. orientale extract were identified. The results showed that 12 metabolites were detected in serum and 26 metabolites in urine( including cross-components) of rats. The main metabolic pathways included hydrogenation,hydroxylation,glucuronidation,sulfation reaction,and methylation-glucuronidation,etc. The method established in this study was reliable and effective for studying the metabolic characteristics of the effective components of P. orientale in rats,and it can provide a reference for further studies on therapeutic material basis of this herb.
采用超高效液相色谱-飞行时间质谱联用（UPLC-Q-TOF-MS/MS）方法分析水红花子有效成分在SD大鼠血清和尿液中的原型及代谢产物。采用Agilent Eclipse Plus C₁₈色谱柱（2.1 mm×100 mm，1.8 μm）进行分离，以0.1%甲酸溶液（A）-乙腈（B）为流动相进行梯度洗脱。在正、负离子电喷雾离子模式下获取生物样品的质谱数据。通过比较空白样品和药物处理样品的色谱差异，鉴定出水红花子提取物有效成分的原型成分和代谢产物。结果表明，大鼠血清中检测到12种代谢产物，尿液中检测到26种代谢产物（包括交叉成分）。主要代谢途径包括氢化、羟基化、葡萄糖醛酸化、硫酸化反应以及甲基化-葡萄糖醛酸化等。本研究建立的方法对于研究水红花子有效成分在大鼠体内的代谢特征可靠有效，可为该药材药效物质基础的进一步研究提供参考。
