Shi Yan, Xiong Jing, Wei Feng, Ma Shuang-Cheng
National Institutes for Food and Drug Control, Beijing 100050, China.
Zhongguo Zhong Yao Za Zhi. 2018 Feb;43(4):651-658. doi: 10.19540/j.cnki.cjcmm.20180105.014.
A LC-MS method was established for study of characteristic ions in frequently-used traditional Chinese medicinal materials derived from animal bile. UPLC-Q-TOF was used in the data acquisition work, then, the software of MarkerLynxTM v.4.1 was performed in the chemometric analysis of data. Besides, selected ion chromatograms of these bile acid ions were comparative studied. Better results were gained in the specificity identification of pig bile, bear bile, cultivated cow-bezoar , artificial cow-bezoar and some compound preparations(Hugan tablet and Rengongniuhuang Jiaxiaozuo capsule) by this method. The method is suitable for the specificity identification of pig bile, bear bile, cultivated cow-bezoar , artificial cow-bezoar and compound preparations containing these medicinal materials.
建立了一种液相色谱-质谱联用（LC-MS）方法用于研究常用动物胆汁类中药材的特征离子。采用超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF）进行数据采集工作，然后运用MarkerLynxTM v.4.1软件对数据进行化学计量学分析。此外，对这些胆汁酸离子的选择离子色谱图进行了比较研究。通过该方法在猪胆汁、熊胆、培植牛黄、人工牛黄及一些复方制剂（护肝片和人工牛黄甲硝唑胶囊）的特异性鉴别中取得了较好的结果。该方法适用于猪胆汁、熊胆、培植牛黄、人工牛黄以及含有这些药材的复方制剂的特异性鉴别。
