Shi Yan, Sun Dong-Mei, Xiong Jing, Wei Feng, Ma Shuang-Cheng
National Institutes for Food and Drug Control, Beijing 100050, China.
Jilin Institute for Food and Drug Control, Jilin 132001, China.
Zhongguo Zhong Yao Za Zhi. 2018 Feb;43(4):659-664. doi: 10.19540/j.cnki.cjcmm.2018.0015.
An HPLC-ELSD method for quantitation of GCANa, CA, HDCA, CDCA and DCA in artificial cow-bezoar was developed in this study. The chromatographic separation was performed using a Wondasil CC₁₈(4.6 mmχ250 mm，5 μm) column at a column temperature of 25 °C and liquid flow-rate of 1.0 mL·min⁻¹. Acetonitrile and 0.2% formic acid solution were used as mobile phase with a linear gradient and injection volume was 10 μL. An ELSD was used with a nitrogen flow-rate of 2.3 L·h⁻¹at a drift tube temperature of 110 °C. Some chemometric methods were applied in data procession and analysis for exploration of the markers of quality difference. The accurate and simple method is suitable for the quality evaluation and the quality control of artificial cow-bezoar.
本研究建立了一种用于定量测定人工牛黄中甘氨胆酸钠、胆酸、猪去氧胆酸、鹅去氧胆酸和脱氧胆酸的高效液相色谱 - 蒸发光散射检测法。色谱分离采用Wondasil C₁₈（4.6 mm×250 mm，5 μm）色谱柱，柱温25℃，液体流速1.0 mL·min⁻¹。乙腈和0.2%甲酸溶液作为流动相进行线性梯度洗脱，进样量为10 μL。采用蒸发光散射检测器，氮气流量为2.3 L·h⁻¹，漂移管温度为110℃。一些化学计量学方法应用于数据处理和分析，以探索质量差异的标志物。该准确且简便的方法适用于人工牛黄的质量评价和质量控制。
