He Hong-Liang, Pan Lin-Li, Gu Xiao-Li, Huang Jiao-Jiao, Sun Cheng-Hong, Tang Yu-Lin, Chen Li-Na
Department of Clinical Pharmacy, Sir Run Run Hospital, Nanjing Medical University, Nanjing 211166, China.
School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
Zhongguo Zhong Yao Za Zhi. 2018 Feb;43(4):748-754. doi: 10.19540/j.cnki.cjcmm.20171208.001.
Magnetic molecularly imprinted polymers(MMIPs) were prepared with ZL006 as template, acrylamide(AA) as the functional monomer, and acetonitrile as pore-forming agent; then Fourier transform infrared spectroscopy(FT-IR) and scanning electron microscopy(SEM) were used to characterize their forms and structures. Simultaneously, the MMIPs prepared previously were used as sorbents for dispersive magnetic solid phase extraction(DSPE) to capture and identify potential nNOS-PSD-95 uncouplers from extracts of Trifolium pratense and the the activities of the screened compounds were evaluated by the neuroprotective effect and co-immunoprecipitation test. The experiment revealed that the successfully synthesized MMIPs showed good dispersiveness, suitable particle size and good adsorption properties. Formononetin, prunetin and biochanin A were separated and enriched from Trifolium pratense by using the MMIPs as artificial antibodies and finally biochanin A was found to have higher cytoprotective action and uncoupling action according to the neuroprotective effect and co-immunoprecipitation test.
以ZL006为模板、丙烯酰胺（AA）为功能单体、乙腈为致孔剂制备了磁性分子印迹聚合物（MMIPs）；然后采用傅里叶变换红外光谱（FT-IR）和扫描电子显微镜（SEM）对其形态和结构进行表征。同时，将先前制备的MMIPs用作分散磁性固相萃取（DSPE）的吸附剂，从红车轴草提取物中捕获和鉴定潜在的nNOS-PSD-95解偶联剂，并通过神经保护作用和共免疫沉淀试验评估筛选出的化合物的活性。实验表明，成功合成的MMIPs具有良好的分散性、合适的粒径和良好的吸附性能。以MMIPs作为人工抗体从红车轴草中分离富集了芒柄花素、樱黄素和鹰嘴豆芽素A，最终根据神经保护作用和共免疫沉淀试验发现鹰嘴豆芽素A具有较高的细胞保护作用和解偶联作用。
